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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Liquid Chromatography/Mass Spectrometry Methods for the Detailed Characterization of Intact Proteins in the Development of Recombinant Glycoprotein Therapeutics
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Liquid Chromatography/Mass Spectrometry Methods for the Detailed Characterization of Intact Proteins in the Development of Recombinant Glycoprotein Therapeutics

机译：液相色谱/质谱法，用于详细表征完整蛋白质在重组糖蛋白治疗剂的开发中

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摘要

Recombinant glycoprotein therapeutics, such as monoclonal antibodies (mAbs), have revolutionized the treatment of a variety of medical ailments, including cancer, autoimmune and musculoskeletal diseases, and hemostatic disorders. Mass spectrometry (MS) is a rapid, highly informative analytical technique capable of detecting structural changes in peptides and proteins with high selectivity and sensitivity, especially when coupled with reversed-phase high-performance liquid chromatography (RP-HPLC). As such, RP-HPLC/MS is an essential tool for the structural characterization of glycoprotein therapeutic candidates, which are often heterogeneous because of post-translational modifications (PTMs) and differential proteolytic processing. Furthermore, RP-HPLC/MS has emerged as a viable technique to demonstrate product comparability, define cell culture and purification processes, and analyze trace-level protein product impurities.
机译：重组糖蛋白治疗剂，例如单克隆抗体（mAb），彻底改变了各种医疗疾病的治疗，包括癌症，自身免疫和肌肉骨骼疾病和止血障碍。质谱（MS）是一种快速高度信息的分析技术，能够检测具有高选择性和敏感性的肽和蛋白质的结构变化，特别是当与反相高效液相色谱（RP-HPLC）相结合时。这样，RP-HPLC / MS是糖蛋白治疗候选物的结构表征的必要工具，其由于翻译后修饰（PTMS）和差分蛋白水解加工而经常是异质的。此外，RP-HPLC / MS已经出现为可行的技术，以证明产品可比性，限定细胞培养和净化过程，并分析痕量蛋白质产物杂质。

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