• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Direct lipids Analyses in Brain Tissue and E. Coli Extract Using Graphite-Assisted Laser Desorption/Ionization Mass Spectrometry
【24h】

Direct lipids Analyses in Brain Tissue and E. Coli Extract Using Graphite-Assisted Laser Desorption/Ionization Mass Spectrometry

机译：使用石墨辅助激光解吸/电离质谱法在脑组织和大肠杆菌提取物中分析脑组织和大肠杆菌的直接脂质分析

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

As imaging mass spectrometry (MS) and microorganism MS have been developed, sample preparation techniques with raw biological materials (tissues, and microorganisms) have become more important. Matrix-assisted laser desoption/ionization(MALDI) MS has been developed for direct tissue lipids analyses recently.1 However, MALDI MS technique for lipids analyses has two main disadvantages. First, matrix co-crystallization process in MALDI MS doesn't provide spot-to-spot reproducibility through the sample surface. This limits the spatial resolution in imaging MS. Second, it has been reported that phosphatidylcholines (PCs) and sphingomyelins(SMs) suppress other lipids signals in positive-ion MALDI MS mode.2 So, here we present alternative sample preparation technique including graphite3,4 coating. With this technique, direct tissue and cell extract lipids analyses were performed.
机译：由于已经开发了成像质谱（MS）和微生物MS，具有原料生物材料（组织和微生物）的样品制备技术变得更加重要。最近已经开发了基质辅助激光脱选/电离（MALDI）MS用于直接组织脂质分析。然而，MALDI用于脂质分析的技术有两个主要缺点。首先，MALDI MS中的基质共结晶方法不能通过样品表面提供点对点再现性。这限制了成像MS中的空间分辨率。其次，据报道，磷脂基胆碱（PC）和鞘磷脂（SMS）抑制正离子MALDI MS模式中的其他脂质信号。如此，我们呈现替代的样品制备技术，包括Graphite3,4涂层。利用这种技术，进行直接组织和细胞提取物脂质分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号