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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Proteomic Analysis of Rat's Cerebrospinal Fluid Changes Following Lipopolysaccharideinduced CNS Inflammation by Liquid Chromatography Tandem Mass Spectrometry
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Proteomic Analysis of Rat's Cerebrospinal Fluid Changes Following Lipopolysaccharideinduced CNS Inflammation by Liquid Chromatography Tandem Mass Spectrometry

机译：通过液相色谱串联质谱法在脂多糖诱导的CNS炎症后改变大鼠脑脊髓液的蛋白质组学分析

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Lipopolysaccharide (LPS) is a constituent in the outer membrane of gram-negative bacteria and is the endotoxin which would induce the inflammation in the central nervous system (CNS). LPS has used to activate microglia to release pro- or anti-inflammatory cytokines, chemokines, prostaglandins, and nitric oxide. Some of these inflammatory molecules are proteins, which may release into the cerebrospinal fluid (CSF). Some CSF proteins known as brainderived proteins are released by some of the CNS cells (e.g., astrocytes and neurons). Characterization of CSF proteome has the potential merit for the understanding of certain biological events in the CNS. The objective of this study was to discover the biological effects of endotoxin lipopolysaccharide (LPS) induced CNS inflammation of rats by intracerebroventricular administration. In this study, we utilized liquid chromatography tandem mass spectrometry (LCMS/ MS) to investigate the changes of the CSF proteome in rats with CNS inflammation. The 5 days after administration was chosen as the CSF sampling time, which could represent the CNS inflammation. 237 different peptides (186 proteins) were identified by LC-MS/MS. Among 186 proteins, there were a total of 18 proteins with significant differences in LPS group (p <0.05), which contained 16 up-regulated and 2 down-regulated. Three proteins: complement C3, platelet-activating factor acetylhydrolase (PAF-Ahs), and serine/threonine-protein kinase MARK2 were reported to be involved in the inflammatory processes in the CNS. In some literatures, it also reported that Lfucose kinase short variant, calcium activated chloride channel, Shen-dan, and CYP2J9 were presented in the CNS. The proteomic profiling of CSF proteins provide not only information on the abundance of various protein contents after treated with LPS but also comprehension for LPS-induced effects on proteins released in the CNS. Keyword: Lipopolysaccharide, cerebrospinal fluid, liquid chromatography tandem mass spectrometry.
机译：脂多糖（LPS）是革兰氏阴性细菌的外膜的组分并且是将诱导炎症在中枢神经系统（CNS）中的内毒素。 LPS已经用于激活的小胶质细胞释放促或抗炎细胞因子，趋化因子，前列腺素，和一氧化氮。一些这些炎性分子是蛋白质，其可以释放到脑脊髓液（CSF）。称为brainderived蛋白一些CSF蛋白通过一些CNS细胞（例如星形胶质细胞和神经元）的释放。 CSF蛋白质的表征具有在中枢神经系统某些生物事件的理解潜在的优点。这项研究的目的是为了发现内毒素脂多糖（LPS）通过脑室注射大鼠的诱导CNS炎症的生物效应。在这项研究中，我们利用液相色谱串联质谱法（LCMS / MS）来调查CSF蛋白质组的大鼠CNS炎症的变化。给药后5天被选为CSF取样时间，这可能代表了CNS炎症。 237种不同的肽（186种蛋白质）通过LC-MS / MS鉴定。间186克的蛋白质，存在总共18种蛋白与在LPS组（p <0.05）差异显著，其中载有16上调和2下调者。三种蛋白：补体C3，血小板活化因子乙酰水解酶（PAF-AHS）和丝氨酸/苏氨酸 - 蛋白激酶MARK2据报道参与CNS中炎症过程。在一些文献中，它也报道Lfucose激酶短变体，钙激活的氯离子通道，沉丹，和CYP2J9分别在CNS中呈现。 CSF蛋白质的蛋白组学不仅提供对大量不同蛋白质含量的信息处理了LPS也是修真LPS诱导对中枢神经系统释放的蛋白质后效果。关键字：脂多糖，脑脊髓液，液相色谱 - 串联质谱法。

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