To discover new serum biomarkers of gastric cancer for early detection. Methods: Surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS) was used to generate proteomic spectra of 32 patients diagnosis as gastric adenocarcinoma, 22 patients with peptic ulcer (PU), 32 patients with gastritis, and 30 normal persons. Then the spectra were analyzed with Ciphergen Biosystem's Software and a new Biomarker Pattern's Software. Results: Five masses with an average mass of 2953, 3267, 5341, 5912, 5927Da up-regulated for gastric cancer from normal persons, and four masses of 4059, 4213, 4270, 7160Da down-regulated. In these masses, 4213, 5341, 5912, 7160Da masses have higher mean peak intensity. Peaks of 4213, 5341, 5912, 7160Da also changed significantly in PU and GS. Peak 5912 is the most interesting protein with a sensitivity of 81.25%, and specificity of 56.67%. Peak 4059 is a small but consistent peak can be clearly seen from control samples but disappears in patient samples. Combination of three biomarkers (4059, 5341, 5912)and (3267, 4059, 5341)were effectively in distinguishing gastric cancer with normal persons. Conclusion: These Low molecular weight serum proteins may be hopeful biomarkers use to detection gastric cancer.
展开▼
