• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >MS Identification of Aspergillus fumigatus T cell Epitopes: A Novel Strategy to Discover Candidate Vaccines that Enhance Cell-mediated Immunity.
【24h】

MS Identification of Aspergillus fumigatus T cell Epitopes: A Novel Strategy to Discover Candidate Vaccines that Enhance Cell-mediated Immunity.

机译：曲霉菌Mumigatus T细胞表位的MS鉴定：一种发现候选疫苗的新策略，增强细胞介导的免疫力。

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aspergillus fumigatus is an opportunistic pathogen that causes invasive aspergillosis in immunocompromised hosts, particularly in patients with hematologic malignancies who undergo allogeneic hematopoietic cell transplantation. Innate immunity plays a key role in the defense against Aspergillus fumigatus in healthy individuals. However, recent evidence suggests that cell-mediated adaptative immunity may provide a critical secondary line of defense against the fungi in immunocompromised individuals. As a result, a vaccine that protects immunocompromised patients is being pursuit. Here, we report a proteomics strategy that utilizes a T cell-based bioassay and mass spectrometry to identify vaccine candidates that may protect the immunosuppressed from invasive aspergillosis. CF1, DBA/2 and BALB/c mice were immunized and boosted with either crude Aspergillus fumigatus extracts or with a recombinant fungal protein, rAsp f 3. Afterwards, mice were immunosuppressed with cortisone and challenged with viable A. fumigatus spores. T cells from lymph nodes and spleens of the survivors of the infection were isolated using magnetic beads. The cells were co-cultured together with autologous irradiated antigen-presenting cells and fungal extracts or rAsp f 3 antigens. To identify T cell epitopes, HPLC fractions of trypsin-digest derived peptides of fungal proteins were analyzed by MALDI-Q-TOF, MALDI-ion trap and LC/FTICR-MS and peptide antigenicities were measured in proliferation assays. Concurrently, we tested the HPLC fractions for the presence of antibody-binding epitopes by ELISA.
机译：烟曲霉是一种条件致病菌引起侵袭性曲菌在免疫受损的宿主，特别是在患有谁接受同种异体造血干细胞移植恶性血液病。先天免疫在针对健康个体烟曲霉防守了关键作用。然而，最近的证据表明，细胞介导的免疫适应性可以在免疫功能低下的个体提供防御的一个重要副线对真菌。其结果是，保护免疫功能低下患者的疫苗是被追求。在这里，我们报告蛋白质组学的策略，利用基于T细胞的生物分析和质谱分析以确定候选疫苗可保护侵袭性曲霉病免疫抑制。 CF1，DBA / 2和BALB / c小鼠进行免疫，并用粗烟曲霉提取物或用重组真菌蛋白升压，锉刀F3。然后，将小鼠用可的松免疫抑制和与活烟曲霉孢子攻击。使用磁珠从淋巴结T细胞和感染的存活者的脾脏分离。将细胞共培养的自体照射的抗原呈递细胞和真菌提取物或锉刀F 3个抗原一起。为了鉴定T细胞表位，的HPLC级分的胰蛋白酶消化的真菌蛋白质的衍生肽通过MALDI-Q-TOF，MALDI-离子阱和LC / FTICR-MS分析和增殖试验测定的肽的抗原性。同时，我们测试了HPLC级分用于通过ELISA的存在抗体结合表位的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号