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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Dual Protease On-line Digestion for High Density Protein Mapping of Large Proteins in Hydrogen/Deuterium Exchange Mass Spectrometry
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Dual Protease On-line Digestion for High Density Protein Mapping of Large Proteins in Hydrogen/Deuterium Exchange Mass Spectrometry

机译：氢气/氘交换质谱中大蛋白的高密度蛋白质测绘的双蛋白酶在线消解

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Pepsin digestion alone generally yielded a sequence coverage of 80-90percent. Combining pepsin and protease pXIII also resulted in a sequence coverage of approximately the same level, but generated different peptides than the pepsin alone. By combining the two sets of data it was possible to achieve an additonal 5-10percent sequence coverage. Even for a relatively small protein such as p38 MAP kinase there can sometimes be regions that are resistant to digestion with pepsin alone. The combination of pepsin/pXIII, while not generating complete coverage of this region did provide several overlapping peptides in this region. The lower sequence coverage for p38 for the pepsin/pXIII vs. pepsin is probably the result of some peptides being digested by the pXIII into fragements too small to be identified.
机译：单独的胃蛋白酶消化通常产生80-90％的序列覆盖率。结合胃蛋白酶和蛋白酶pXIII也导致序列覆盖率大致相同的水平，但是产生不同的肽而不是百事可乐。通过组合两组数据，可以实现附加的5-10percent序列覆盖范围。即使对于相对小的蛋白质，例如P38 MAP激酶，有时也可以是单独用胃蛋白酶消化的区域。 Pepsin / PXIII的组合，同时没有产生该地区的完全覆盖确实在该地区提供了几种重叠肽。对于Pepsin / PXIII与百事量的p38的序列覆盖率较低可能是通过PXIII消化的一些肽变成太小而无法识别的片段的结果。

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