Simultaneous DNA, RNA, and Protein Extraction from Microbial Cells: Sequential Isolation of Complex Biomolecules from a Single Source

机译：同时DNA，RNA和蛋白质从微生物细胞中提取：从单一来源顺序分离复杂生物分子

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High quality gDNA, total RNA and proteins were efficiently extracted from a single starting sample with consistent yields and high purities, regardless of microbial cell wall structure. Total RNA had the highest measurable integrity and included mRNA and other small RNA species. The spin filter purified sample was not appreciably different in the number of identified proteins or functional group classifications compared to unpurified and sodium carbonate extracted proteins. Correlations between DNA,RNA, and proteins can be made within a single starting sample.

机译：无论微生物细胞壁结构如何，高质量的GDNA，总RNA和蛋白质从单个起始样品中有效地从单一的起始样品中提取。总RNA具有最高的可测量完整性，包括mRNA和其他小RNA物种。与未纯化和碳酸钠提取的蛋白质相比，旋转过滤器纯化样品在鉴定的蛋白质或官能团分类的数量中没有明显不同。 DNA，RNA和蛋白质之间的相关性可以在单一的起始样品中制备。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2016年|1CD|共1页
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H.A. Callahan; V. Nieciecki; E. Adams;
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中图分类 TB994.5-53;
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2. SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells [J] . Han Kyung Yeon, Kim Kyu-Tae, Joung Je-Gun, Genome research . 2018,第1期

机译：SIDR：单细胞基因组DNA和总RNA的同时分离和平行测序
3. Direct DNA Isolation from Solid Biological Sources without Pretreatments with Proteinase-K and/or Homogenization through Automated DNA Extraction [J] . Jang-Seu Ki, Ki Byum Chang, Hee June Roh Journal of Bioscience and Bioengineering . 2007,第3期

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4. Simultaneous DNA, RNA, and Protein Extraction from Microbial Cells: Sequential Isolation of Complex Biomolecules from a Single Source [C] . H.A. Callahan, V. Nieciecki, E. Adams ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：同时DNA，RNA和蛋白质从微生物细胞中提取：从单一来源顺序分离复杂生物分子
5. Isolation and functional mapping of Human T-cell Leukemia Virus Type 1 Tax oncoprotein DNA-damage complexes. [D] . Durkin, Sarah Saionz. 2006

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6. SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells [O] . Kyung Yeon Han, Kyu-Tae Kim, Je-Gun Joung, 2018

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7. The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivided mixed microbial community sample [O] . Emilie MULLER, Emilie Muller, Anna Heintz-Buschart, 2014

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Simultaneous DNA, RNA, and Protein Extraction from Microbial Cells: Sequential Isolation of Complex Biomolecules from a Single Source

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