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首页> 外文会议>International Mass Spectrometry Conference >Imaging mass spectrometry in biological tissues by laser ablation inductively coupled plasma mass spectrometry
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Imaging mass spectrometry in biological tissues by laser ablation inductively coupled plasma mass spectrometry

机译：激光烧蚀电感耦合等离子体质谱法在生物组织中成像质谱

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Of all the inorganic mass spectrometric techniques, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) plays a key role as a powerful and sensitive microanalytical technique enabling multi-element trace analysis and isotope ratio measurements at trace and ultratrace level. LA-ICP-MS was used to produce images of detailed regionally-specific element distribution in 20 (mu)m thin sections of different parts of the human brain. The quantitative determination of copper, zinc, lead and uranium distribution in thin slices of human brain samples was performed using matrix-matched laboratory standards via external calibration procedures. Imaging mass spectrometry provides new information on the spatially inhomogeneous element distribution in thin sections of human tissues, for example, of different brain regions (the insular region) or brain tumor tissues. The detection limits obtained for Cu, Zn, Pb and U were in the ng g~(-1) range. Possible strategies of LA-ICP-MS in brain research and life sciences include the elemental imaging of thin slices of brain tissue or applications in proteome analysis by combination with matrix-assisted laser desorption/ionization MS to study phospho- and metal-containing proteins will be discussed.
机译：所有的无机质谱技术，激光烧蚀电感耦合等离子体质谱法（LA-ICP-MS）起着作为一个强大的和敏感微量分析技术使多元素微量分析和同位素比率测量在跟踪和超痕量水平中起关键作用。 LA-ICP-MS用于在20（亩）详述的特定区域的元素分布的产生图像M人类大脑的不同部位的薄切片。通过外部校准程序使用基体匹配的标准实验室进行的铜，锌，铅和铀分布在人脑样品的薄片的定量测定。成像质谱提供在人体组织中的薄切片的空间不均匀元素分布的新信息，例如，不同的脑区域（岛状区域）或脑肿瘤组织。对Cu，锌，铅和U获得的检测限为毫微克克〜（-1）的范围。在脑研究和生命科学LA-ICP-MS的可能的策略包括通过用基质辅助激光解吸/电离MS到研究磷酸和含金属的蛋白质组合在蛋白质组分析脑组织或应用程序的薄片的元素成像将进行讨论。

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