• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Mass Spectrometry Conference >DEVELOPMENT AND VALIDATION OF A HIGH SENSITIVITY ASSAY OF ESTROGENS IN HUMAN PLASMA BY UHPLC-MS/MS WITHOUT DERIVATIZATION
【24h】

DEVELOPMENT AND VALIDATION OF A HIGH SENSITIVITY ASSAY OF ESTROGENS IN HUMAN PLASMA BY UHPLC-MS/MS WITHOUT DERIVATIZATION

机译：UHPLC-MS / MS没有衍生化的UHPLC-MS / MS在人血浆中雌激素高敏感性测定的开发和验证

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Estrogens play important roles in many biological functions as primary female sex hormones. In males, estrogens are present at lower concentrations but are also involved in several mechanisms. Modification of circulating estrogen levels can be associated with both positive and negative health effects. In the last decade, LC/MS/MS has proven its capabilities in terms of sensitivity and specificity to become the gold standard methodology in steroid analysis. However, measuring estrogens at very low level remained challenging. Many published methods involve complex sample preparation, large sample volumes or derivatization. These process are not compatible with a routine laboratory workflow. Here we present a UHPLC-MS/MS method to measure estrone (E1), estradiol (E2) and estriol (E3) together in the low pg/mL range.
机译：雌激素在许多生物学功能中发挥重要作用作为原发性女性性激素。在雄性中，雌激素以较低浓度存在，但也参与了几种机制。循环雌激素水平的改性可以与阳性和阴性健康效应相关。在过去的十年中，LC / MS / MS在敏感性和特异性方面证明了其能力，成为类固醇分析中的黄金标准方法。然而，测量在极低水平下的雌激素仍然具有挑战性。许多公开的方法涉及复杂的样品制剂，大样本量或衍生化。这些过程与日常实验室工作流程不兼容。在这里，我们提出了一种UHPLC-MS / MS方法来测量雌激素（E1），雌二醇（E2）和雌二醇（E3）在低pG / mL范围内。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号