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首页> 外文会议>General Meeting of European Society of Animal Cell Technology >Acoustic Cell Processing for Viral Transduction or Bioreactor Cell Retention
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Acoustic Cell Processing for Viral Transduction or Bioreactor Cell Retention

机译：用于病毒转导或生物反应器细胞保留的声学电池处理

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A major limitation of retro virus gene therapy technology is the often low percentage of target cells transduced. This is in part due to the low diffusivity of retroviruses, as well as their short half-life (~5 h). An approach to overcome these limitations has been developed using the forces in an acoustic standing wave field. An air-backflush mode of operation obtained up to 8-fold increases in TF-1 cell transduction compared to static controls and this was sustained from 2 to 24 h. The transductionincreased as a function of power input, but at elevated power levels the acoustic transducer generated excessive heat. A new design with improved heat dissipation allowed continuous acoustic treatment over 2 days with no decrease in cell viability. Thisacoustically increased transduction reduces the need for additives and avoids the complications of recovering anchored cells. While acoustic separators can be used for bioreactor volumes ranging from hundreds of mL to > 100 L, it is also important to define operational settings that avoid negative thermal influences on the cells. Additional cell culture experiments with CHO cells were performed to determine the acceptable temperature variations.
机译：复古病毒基因治疗技术的主要限制是转导的靶细胞的常见百分比。这部分是由于逆转录病毒的低扩散性，以及它们的短半衰期（〜5小时）。使用声学驻波场中的力来开发了一种克服这些限制的方法。与静态对照相比，在TF-1细胞转导中获得的空气反吹术模式可达8倍，并且这持续2至24小时。作为电源输入的功能，转导仪增加，但在电力水平升高时，声学换能器产生过多的热量。具有改善的散热性的新设计允许连续的声学处理超过2天，不会降低细胞活力。这种声法增加的转导减少对添加剂的需要，并避免回收锚固细胞的并发症。虽然声学分离器可用于从数百毫升到> 100L的生物反应器容积，但对于限定避免对细胞的负热影响的操作设置也很重要。进行额外的细胞培养实验与CHO细胞进行测定以确定可接受的温度变化。

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