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首页> 外文会议>Australian cereal chemistry conference >ANALYSIS OF HETEROLOGOUS PROMOTERS IN TRANSGENIC RICE
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ANALYSIS OF HETEROLOGOUS PROMOTERS IN TRANSGENIC RICE

机译：转基因水稻异源启动子分析

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The genomes of cereals are one of the many that humans have remodelled through careful selection and breeding-criteria such as high yield, disease tolerance or environmental adaptability. Biotechnology offers another avenue for modification of genomesby transferring one or more transgenes for end-use quality applications or the use of plants as bio factories for the manufacture of various products. The economics of using cereal grain for the production of bio-molecules would depend on value of the bio-molecule and the amount synthesised in the grain. Several reports demonstrate that rice grains can be used for end-use quality or as a production platform for seed-based edible vaccines. Beta-carotene was produced in the rice grain with the aim of alleviating Vitamin A deficiency in human populations (Paine et al., 2005), and rice grains have also been used as a platform for the production of vaccines (Takaiwa et al., 2007; Wu et al., 2007). To maximise the benefits of this technology it would be highly desirable to generate transgenic rice with multi-traits (gene stacking) conferred by many genes under control of as many promoters. However, use of the same promoter to drive more than one transgene, can result in impaired expression of one or more transgenes through a process of homologous sequence-dependent gene silencing (transcriptional silencing) (Butaye et al., 2005; Jorgensen, 1992; Rocha et al, 2005). The need to stack more than one trait in the same seed tissue of rice will require the useof homologous or heterologous promoters with the same tissue specificities but with little homology.
机译：谷物的基因组是通过仔细选择和育种标准进行改造的许多人之一，例如高产，疾病耐受性或环境适应性。 Biotechnology提供了另一种用于改性Genomesby的途径，转移一个或多个转基因用于最终用途的质量应用或使用植物作为各种产品制造的生物工厂的使用。使用谷粒生产生物分子的经济学将取决于生物分子的价值和在晶粒中合成的量。几份报告表明，稻粒可用于最终使用质量或作为种子可食用疫苗的生产平台。 β-胡萝卜素在水稻谷物中产生，目的是减轻维生素的人口缺乏（Paine等，2005），而水稻颗粒也被用作生产疫苗的平台（Takaiwa等， 2007; Wu等，2007）。为了最大化该技术的益处，非常希望产生通过在许多启动子的许多基因的多种基因赋予的多重性（基因堆叠）产生转基因水稻。然而，使用相同的启动子来驱动多于一个的转基因，可导致通过同源序列依赖性的基因沉默（转录沉默）的过程中的一个或多个转基因的表达受损（Butaye等人，2005;乔根森，1992; Rocha等，2005）。在稻米的同一种子组织中堆叠多于一个特征的需要将需要使用同源或异源启动子以相同的组织特异性，但具有很少的同源性。

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