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首页> 外文会议>Australian cereal chemistry conference >PHYSICAL INTERACTION OF NATIVE AND MUTATED FORMS OF PUROINDOLINES SHOWN USING THE YEAST TWO HYBRID SYSTEM
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PHYSICAL INTERACTION OF NATIVE AND MUTATED FORMS OF PUROINDOLINES SHOWN USING THE YEAST TWO HYBRID SYSTEM

机译：使用酵母两种杂交系统所示的天然和突变形式的嘌呤吲哚的物理相互作用

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Grain hardness is an important trait used in the classification of wheat and in determining its end use. Two major components of a protein group associated with the surface of starch granules, puroindoline a (PINA) and puroindoline b (PINB), have beento shown to significantly influence grain hardness. Common wheats that have both puroindolines present in their wild form are soft textured, while the various hard kernel phenotypes have mutation or deletion in either PINs. The extremely hard durum wheat lacks these proteins. The primary role for these lipid binding proteins is most likely antimicrobial, as demonstrated in previous in-vitro and in-vivo studies (Bhave and Morris, 2008). Several reports on transcripts and protein level comparison as wellas transgenic work, have shown that the lipid binding properties of PINs require some co-operation or interdependence (Capparelli et al, 2003: Amoroso et al, 2004; Hogg et al, 2004; Swan et al, 2006). An understanding of the interactions between these proteins would help elucidate their biological function in grain texture and possible antimicrobial applications. In this study, the yeast two hybrid system was used to study physical interactions between PINA and PINB from two soft common wheats and a Pinb-Dlb wheat type.
机译：谷物硬度是小麦分类和确定其最终用途的重要特征。与淀粉颗粒表面相关的蛋白质组的两个主要组分，嘌呤啉甲脒A（PINA）和嘌呤吲哚B（PINB），具有显示为显着影响晶粒硬度的Beento。具有其野生形式存在的两个嘌呤吲哚吲哚的常见小麦是柔软的纹理，而各种硬核表型在任何一个引脚中都具有突变或缺失。极其硬的硬质小麦缺乏这些蛋白质。这些脂质结合蛋白的主要作用是最可能的抗微生物，如前体外和体内研究（Bhave和Morris，2008）所示。几次关于转录物和蛋白质水平对比的报告和蛋白质水平的比较表明，别针的脂质结合特性需要一些合作或相互依存（Capparelli等，2003：Amoroso等，2004; Hogg等，2004; Swan et al，2006）。理解这些蛋白质之间的相互作用将有助于阐明其在谷物质地和可能的抗微生物应用中的生物学功能。在这项研究中，酵母两种杂交系统用于研究Pina和Pinb之间的物理相互作用，从两个柔软的常见小麦和销DLB小麦型。

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