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Evaluating the Geochemical and Microbial Response to ERH and Implications for Bioremediation

机译:评估地球化学和微生物反应对ERH的影响及其对生物修复的影响

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The complexity of sites contaminated with chlorinated solvent dense nonaqueous phase liquids (DNAPLs) in groundwater has led to evaluation of multi-component reme- dies. Relatively high cost, aggressive treatments, such as electrical resistance heating (ERH), can remove large quantities of DNAPL, and may be more cost-effective when used in conjunction with low-cost, passive treatments, such as bioremediation, to reduce dissolved phase contaminants to regulatory compliance levels. The Ft. Lewis East Gate Disposal Yard (EGDY) contains a residual source area containing trichloroethene DNAPL, which was the site of a year-long enhanced bioremediation demonstration using whey powder resulting in the enrichment of an efficient population of Dehalococcoides spp. (DHC) containing all three reductive dehalogenase genes tceA, bvcA and vcrA, and capable of complete reductive dechlorination to ethene. Seven months following the demonstration, ERH began, as the final remedy, but high concentrations of DHC remained as did ethene. The focus of this study was to evaluate the effect of heating and cooling associated with ERH on the prevalent microbial community by sampling before, during, and after heating. In addition, biological attenuation of contaminants at different temperatures was evaluated by integrating microbial, molecular data with groundwater geochemical and contaminant data.
机译:地下水中含有氯化溶剂致密非水相液(DNAPLS)污染的部位的复杂性导致了对多组分的腐蚀性的评价。相对较高的成本,腐蚀性处理,例如电阻加热(ERH),可以去除大量的DNAPL,并且当与低成本,无源处理(例如生物修复)结合使用时,可以更具成本效益,以减少溶解相污染物到监管依从性水平。 ft。刘易斯东门处理码(egdy)含有含有三氯乙烯DNAPL的残留源区,是使用乳清粉的一年增强的生物修复示范的位点,从而富集的脱卤素群体的富集。 (DHC)含有所有三种还原脱色酶基因TCEA,BVCA和VCRA,并能够完全将脱氯完全脱乙烯。示威后七个月,ERH开始作为最终的补救措施,但高浓度的DHC仍然和乙烯一样。本研究的重点是通过在加热之前,期间和之后进行采样来评估与普遍的微生物群落中的加热和冷却与IRH相关的影响。此外,通过将微生物的分子数据与地球化学和污染物数据集成来评估不同温度下的污染物的生物衰减。

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