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首页> 外文会议>European Meeting on Supercritical Fluids >PRODUCING CHITIN AND CHITIN-GLUCAN COMPLEXES FROM ASPERGILLUS NIGER BIOMASS USING SUBCRITICAL WATER
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PRODUCING CHITIN AND CHITIN-GLUCAN COMPLEXES FROM ASPERGILLUS NIGER BIOMASS USING SUBCRITICAL WATER

机译：使用亚临界水从Aspergillus尼日尔生物量生产甲壳素和甲壳素 - 葡聚糖复合物

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Chitin is the second most abundant biopolymer on earth, occurring in marine invertebrates, insects, and the cell walls of some fungi, among them Aspergillus niger, which is used in the industrial production of citric acid. Such biopolymer and its derivatives have a broad range of applications in many economically relevant areas (biomedical, food, agriculture, paper, wastewater treatment, pharmaceutical, etc.). However, the conventional process for obtaining chitin uses elevated amounts of sodium or potassium hydroxide, and thus has difficulties associated with its environmental performance. In this work we explored a process for obtaining chitin and chitin-glucan complexes from mycelium of Aspergillus niger by treatment with subcritical water. A continuous apparatus designed for operation at temperatures up to 300°C, pressures up to 29 MPa and residence times of mycelium from 0.4 to 7.5 s was built. A central composite design was run to investigate the effect of temperature and reaction time over the composition and properties of the product, such as yield, molecular weight and chitin concentration. With this process a variety of products can be obtained. For example, at 223°C, 21 MPa and 1.6 s residence time, a chitin-glucan complex is obtained, with a yield of 53% (based on the dry mycelium fed), a chitin concentration of 30% and a molecular weight of 21.2 kDa. This product might be useful as a raw material for biomedical products of high value. The developed process is promising for industrial production since it only uses the most abundant, inexpensive and environmentally friendly solvent: water.
机译：几丁酸是地球上最丰富的生物聚合物，在海洋无脊椎动物，昆虫和一些真菌的细胞壁中发生，其中曲霉尼日尔，用于工业生产的柠檬酸。这种生物聚合物及其衍生物在许多经济相关地区（生物医学，食品，农业，造纸，废水处理，制药等）中具有广泛的应用。然而，用于获得甲壳素的常规方法使用升高的钠或氢氧化钾，因此难以与其环境性能相关的困难。在这项工作中，我们探讨了通过用亚临界水的处理来获取从烟草菌丝菌丝菌丝体菌丝菌丝菌丝和甲壳素 - 葡聚糖复合物的过程。在温度温度下设计的连续装置，高达29MPa的压力和菌丝的停留时间为0.4至7.5秒。运行中央复合设计以研究温度和反应时间对产物的组成和性质的影响，例如产率，分子量和几丁质浓度。通过该过程，可以获得各种产品。例如，在223℃，21MPa和1.6秒的停留时间下，获得三蛋白 - 葡聚糖络合物，产率为53％（基于干菌丝菌塞菌丝菌塞），几丁质浓度为30％和分子量21.2 KDA。该产品可用作高价值的生物医学产品的原料。开发的过程是对工业生产的承诺，因为它只使用最丰富，最便宜和环保的溶剂：水。

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