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Genotypic and symbiotic characters among rhizobia nodulating red clover in soils of northern Scandinavia

机译:斯堪的纳维亚北部土壤中的根瘤菌和分解性与共生特征

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Indigenous populations ofRhizobium leguminosarum bv. trifolii nodulating red clover (Trifo-lium pratense L.) were sampled from two field sites, in northern Sweden and northern Norway, at different times of the growing season. A total of 431 nodule isolates were characterized genetically by targeting both chromosomal and symbiotic genes. The chromosomal analysis revealed considerable genetic variation within the isolated populations that was more influenced by geographical origin than sampling time. Restriction fragment length polymorphism analysis of the nodEF gene on the symbiotic plasmid revealed a high proportion of nod genotypes common to the two sites. The symbiotic efficiency of eight isolates, representing both dominating and rare nod genotypes, was measured in a greenhouse experiment using the ~(15)N isotope dilution method. All tested isolates except one, representing a very rare genotype, showed high N_2 fixation rates in symbiosis with the host plant. There was thus no correlation between relative abundance and symbiotic efficiency among the sampled rhizobial genotypes, and we conclude that effective N_2-fixing strains of Rhtobium leguminosarum bv. trifolii nodulating red clover are common and genetically diverse in these northern Scandinavia soils.
机译:土着植物植物杆菌菌。在瑞典北部和挪威北部,在不断增长的季节的不同时期,Trifolii向瑞典和挪威北部的两个田地场所取样。通过靶向染色体和共生基因,总共431个结节分离物在遗传上表征。染色体分析揭示了在分离的群体内的相当大的遗传变异,这些群体在地理来源的影响比采样时间更大。结核病对共生质粒的限制片段长度多态性分析显示了两种位点常见的高比例的NOD基因型。在使用〜(15)n同位素稀释方法的温室实验中测量了八个分离株的共生效率,其占主导地位和稀有的NOD基因型。除了一个代表一个非常罕见的基因型的所有测试分离物,显示出与宿主植物共生的高NO_2固定率。因此,采样的Rhizobial基因型之间的相对丰富和共生效率之间没有相关性,我们得出结论,rhtobium乳糜序曲BV的有效N_2固定菌株。在斯堪的纳维亚土壤中,Trifolii标注红三叶草是常见的,遗传多样化。

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