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首页> 外文会议>International symposium on plant-soil interactions at low pH >Denitrifying Enzyme Activity in Acidic Arable Soils: Effects of Antecedent Water Regimes and Chloramphenicol
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Denitrifying Enzyme Activity in Acidic Arable Soils: Effects of Antecedent Water Regimes and Chloramphenicol

机译：酸性耕作土壤中的硝化酶活性：前一种水分和氯霉素的影响

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A short-term anaerobic assay with or without 0.1 g/L chloramphenicol was used to determine the denitrifying enzyme activity (DEA) of two paddy and two upland red soils. The four soils were stored field-moist at ～4°C ( sample F) , maintained at a soil water content of 40% water holding capacity (sample M) , or saturated with distilled water (sample S) for 110 days. The DEA (μg N2O-N kg-1 h-1) ranged from 2. 15 to 18. 7 in upland soils, and 6. 26 to 327 in paddy soils. For upland soils, submergence increased DEA both with and without the presence of chloramphenicol in the assay ( P <0.01). For paddy soils submergence decreased DEA when chloramphenicol was used, and increased DEA when chloramphenicol was not used (P < 0.05). For the M samples, there were significant differences in the DEA assay with and without choloramphenicol ( P < 0.05 ), but the difference was insignificant for the S samples.
机译：使用或不含0.1g / L氯霉素的短期厌氧测定法测定两种稻草和两个旱红色土壤的反硝化酶活性（DEA）。将四个土壤储存在〜4℃（样品F）的静脉湿，保持在40％水持续容量（样品M）的土壤含水量（样品M），或用蒸馏水（样品）饱和110天。 DEA（μgN2O-N kg-1 H-1）的范围为2. 15至18.7在旱地土壤中，6.6至327个稻田土壤。对于高地土壤，淹没在测定中没有氯霉素的情况增加了DEA（P <0.01）。对于水稻土壤淹没，使用氯霉素时，DEA下降，并且不使用氯霉素时的DEA增加（P <0.05）。对于M样品，DEA测定有显着差异，无胆胺蛋白（P <0.05），但S样品差异是微不足道的。

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