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The Construction and Characterization of Self-sufficient Sterol 14-Demethylase Fusion Proteins consisting of Yeast CYP51 and NADPH-450 reductase

机译：由酵母CYP51和NADPH-450还原酶组成的自给式甾醇14-脱甲基酶融合蛋白的构建与表征

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We constructed two forms of catalytically active fused enzyme consisting of yeast CYP51 (lanosterol 14-demethylase) and yeast NADPH- P450 reductase (P450 reductase) to construct a self-sufficient C-C bond cleaving P450.Two fused enzymes named F1 and F2 having different linkers between the CYP51 and the reductase domains were expressed in E. coli cells and purified to homogeneity. The purified F1 and F2 preparations showed spectral properties typical for fused proteins having both P450 and flavoprotein. F1 and F2 showed lanosterol 14-demethylase activity com- parable to that of the reconstituted system with purified CYP51 and P450 reductase. The rates of the enzymatic reduction of the P450 in F1 and F2 were somewhat faster than that observed on the reconstituted system.

机译：我们构建了由酵母CYP51（Lanterol醇14-脱甲基酶）和酵母NADPH-P450还原酶（P450还原酶）组成的两种形式的催化活性融合酶，以构建自给自足的CC键裂解P450.TWO命名为F1和F2具有不同接头的酶在CYP51和还原酶结构域之间在大肠杆菌细胞中表达并纯化为均匀性。纯化的F1和F2制剂显示出具有P450和黄烷烃蛋白的熔融蛋白的典型光谱性能。 F1和F2显示Lanteroll 14-脱甲基酶活性与纯化的CYP51和P450还原酶的重构系统相比。 F1和F2中P450的酶促减少的速率略微比在重构系统上观察到的速度。

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《International Conference on Cytochrome P450》|2009年||共4页
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Kitahama Y.; Nakamura M.; Yoshida Y. Aoyama Y;
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1. The Construction and Characterization of Self-Sufficient Lanosterol 14-Demethylase Fusion Proteins Consisting of Yeast CYP51 and Its Reductase [J] . Kitahama Y, Nakamura M, Yoshida Y, Biological & pharmaceutical bulletin . 2009,第4期

机译：酵母CYP51及其还原酶组成的自足羊毛甾醇14-脱甲基酶融合蛋白的构建及鉴定
2. Expression and Characterization of CYP51, the Ancient Sterol 14-demethylase Activity for Cytochromes P450 (CYP), in the White-Rot Fungus Phanerochaete chrysosporium [J] . Andrew Warrilow, Cynthia Ugochukwu, David Lamb, Lipids . 2008,第12期

机译：CYP51的表达和特征，CYP51，在细胞色素P450（CYP）中的古老甾醇14-脱甲基酶活性，在白腐真菌Phanerochaete chrysporporium中
3. Expression and Characterization of CYP51, the Ancient Sterol 14-demethylase Activity for Cytochromes P450 (CYP), in the White-Rot Fungus Phanerochaete chrysosporium. [J] . Warrilow A, Ugochukwu C, Lamb D, Lipids . 2008,第12期

机译：CYP51的表达和特征，CYP51在白腐真菌Phanerochaete chrysosporium中对细胞色素P450（CYP）的古代甾醇14-脱甲基酶活性。
4. The Construction and Characterization of Self-sufficient Sterol 14-Demethylase Fusion Proteins consisting of Yeast CYP51 and NADPH-450 reductase [C] . Kitahama Y., Nakamura M., Yoshida Y. Aoyama Y International Conference on Cytochrome P450 . 2009

机译：由酵母CYP51和NADPH-450还原酶组成的自给式甾醇14-脱甲基酶融合蛋白的构建与表征
5. Construction of lactose-fermenting and high-ethanol-producing hybrid yeast by protoplast fusion of Saccharomyces cerevisiae and Kluyveromyces fragilis and the analysis of beta-galactosidase gene in the hybrid yeast. [D] . Farahnak, Fereydoon. 1988

机译：通过酿酒酵母和脆弱克鲁维酵母的原生质体融合构建乳糖发酵和高产乙醇的杂交酵母，并分析该杂交酵母中的β-半乳糖苷酶基因。
6. Characterization of Saccharomyces cerevisiae CYP51 and a CYP51 fusion protein with NADPH cytochrome P-450 oxidoreductase expressed in Escherichia coli. [O] . K Venkateswarlu, D E Kelly, S L Kelly 1997

机译：酿酒酵母CYP51和一种CYP51融合蛋白与在大肠杆菌中表达的NADPH细胞色素P-450氧化还原酶的鉴定。
7. The Construction and Characterization of Self-Sufficient Lanosterol 14-Demethylase Fusion Proteins Consisting of Yeast CYP51 and Its Reductase [O] . Yutaka Kitahama, Masashi Nakamura, Yuzo Yoshida, 2009

机译：由酵母CYP51组成的自给式Lanterol醇14-脱甲基酶融合蛋白的构建与表征及其还原酶
8. Cloning and Characterization of Genes Encoding Proteins Involved in the TerminalStage of Vesicular Traffic in the Yeast 'Saccharomyces cerevisiae' [R] . Aalto, M. 1995

机译：酵母'酿酒酵母'中水泡交通终末期蛋白质编码基因的克隆与鉴定

The Construction and Characterization of Self-sufficient Sterol 14-Demethylase Fusion Proteins consisting of Yeast CYP51 and NADPH-450 reductase

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