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Nanotechnology for visualization, counting and kinetic studies of cytochrome P450 complexes

机译：用于可视化，计数和细胞色素P450络合物的计数和动力学研究的纳米技术

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AFM optico- and optico-acoustic biosensors were used for detection structural and molecular organization of protein-partner complexes in water-soluble and membrane P450-containing monooxygenase systems. AFM-molecular detector was used for detection, visualization and direct counting of proteins, binary and ternary protein complexes forming in P450101, P4502B4 and P45011A1 - containing systems. With membrane P4502B4 and P45011A1 -systems, the membrane proteins were monomerized in solution by Emulgen 913.Using the optical two-channel biosensor "resonant mirror", the IAsys kinetic constants of formation and decay of protein-partners complexes were measured from the ratio of which the values of Kd for reactions of complex formation in three P450-containing systems were calculated. Comparison of the life time of complexes with the time of catalytic cycle showed that in P450cam and P45011A1systems the ternary complexes are productive while the binary ones are deadlocked. In P4502B4 the both binary and 30% of ternary complexes are productive.
机译：AFM Optico和Optico-acoustic Biosensors用于检测水溶性和含膜P450的单氧化酶系统中蛋白质合作蛋白合综合体的结构和分子组织。 AFM分子检测器用于P450101，P4502B4和P45011A1的系统中形成的蛋白质，二元和三元蛋白复合物的检测，可视化和直接计数。通过膜P4502B4和P45011A1 - 系统，通过Emulgen 913在溶液中单体化。使用光学双通道生物传感器“共振镜”，从属于的比例测量蛋白质 - 合作伙伴复合物的形成和衰减的IASYS动力学常数。计算了三种P450系统中复杂形成反应的Kd的值。催化循环时间的复合物的寿命比较显示，在P450CAM和P45011A1系统中，三元复合物在二元复合物的僵化中具有效力。在P4502B4中，二元和30％的三元复合物都是生产性的。

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