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首页> 外文会议>American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics >The stationary phase response regulator SprE (RssB) is involved in the regulation of polyadenylation and mRNA stability in Escherichia coli: in vivo evidence for PAP I interaction with the degradosome
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The stationary phase response regulator SprE (RssB) is involved in the regulation of polyadenylation and mRNA stability in Escherichia coli: in vivo evidence for PAP I interaction with the degradosome

机译：静止阶段响应调节器SPRE（RSSB）参与了大肠杆菌中的聚腺苷酸化和mRNA稳定性的调节：在体内证据表明PAP I与降解组合相互作用

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Prokaryotes use alternate sigma factors of RNA polymerase for transcriptional selectivity in response to different stresses. rpoS encodes the stationary phase sigma factor, (sigma)~(S), which is required for proper expression of about 100 stress-response genes. When nutrients are abundant, SprE binds RpoS and delivers it to ClpXP for degradation, but when nutrients are absent this process is inhibited. Overproduction of SprE causes a toxicity phenotype, seen as a severe growth defect. Based on the known function of SprE, it is not inherently obvious of how this protein could be toxic. Null mutations in pcnB [encoding poly(A) polymerase I (PAP I)] and hfq were found to suppress the toxicity phenotype. PAP I in conjunction with the RNA chaperone Hfq adds poly(A) tails onto the 3' ends of mRNAs which target them for destruction. Here we use biochemical assays to demonstrate that SprE has a second function involved in the PAP I polyadenylation pathway and hence control of mRNA stability. We show that PAP I interacts with the degradosome in both growth phases, and this interaction is dependent upon SprE during stationary phase.
机译：原核生物使用RNA聚合酶的替代Sigma因子进行转录选择性，响应于不同的应力。 RPO编码固定阶段Sigma因子（Sigma）〜（s），这是适当表达约100个应激响应基因所必需的。当营养素丰富时，SPRE结合RPO并将其递送给CLPXP进行降解，但当营养素不存在时，该过程受到抑制。 SPRE过量导致毒性表型，被视为严重的生长缺陷。基于SPRE的已知功能，本蛋白是如何有毒的。发现PCNB中的突变[编码聚（a）聚合酶I（PAP I）]和HFQ抑制毒性表型。 PAP I与RNA伴侣HFQ一起添加到MRNA的3'末端的聚（a）尾部，其针对销毁。在这里，我们使用生化测定来证明SPRE具有涉及PAP I聚腺苷酸化途径的第二个功能，从而控制mRNA稳定性。我们表明PAP I与生长阶段的降解组相互作用，并且这种相互作用取决于固定阶段的SPRE。

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