MASS SPECTROMETRIC ANALYSIS OF POLYKETIDE BIOSYNTHESIS: FTICR-MS VERSUS LOW-RESOLUTION LC-MS FOR ANALYSIS OF ACTIVE-SITE BOUND INTERMEDIATES

机译：聚酮化合物生物合成的质谱分析：FTICR-MS与低分辨率LC-MS分析，用于分析有源点结合的中间体

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1. Direct inject FTICR MS allows for many samples to be run rapidly, allowing for rapid kinetic characterization of PKS systems. (PikAIV AT only). 2. LC-FTICR MS can assist in developing a model for PKS biosynthesis by monitoring ACP active site bound intermediates, which are difficult to monitor. 3. Low resolution LC-qMS can be used in conjunction with a high-resolution, MS/MS instrument (eg. real world concerns about instrument time). 4. Low resolution LC-qMS offers suprisingly good spectra of intact proteins. 5. Combining complimentary MS methods will allow for a model of PKS biosynthesis to be developed.

机译：1.直接注射FTICR MS允许迅速运行许多样品，从而允许PKS系统的快速表征。（只有Pikaiv）。 2. LC-FTICR MS可以通过监测ACP活性位点中间体来帮助开发PKS生物合成的模型，这难以监测。 3.低分辨率LC-QM可以与高分辨率，MS / MS仪器配合使用（例如，真实世界担心仪器时间）。 4.低分辨率LC-QMS提供满意的完整蛋白质的良好光谱。 5.结合免费MS方法将允许开发PKS生物合成的模型。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2009年||共1页
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Christopher M. Rath; Shilah Bonnet; Kevin Renoylds; David H. Sherman; Kristina Hakansson;
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MASS SPECTROMETRIC ANALYSIS OF POLYKETIDE BIOSYNTHESIS: FTICR-MS VERSUS LOW-RESOLUTION LC-MS FOR ANALYSIS OF ACTIVE-SITE BOUND INTERMEDIATES

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