An In-Depth Parallel Study on Using Various MALDI Matrices for MicroRNA Analysis

机译：使用各种MALDI矩阵进行MicroRNA分析的深度平行研究

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50percent DMF was confirmed to provide sufficient solubility for each MALDI matrix up to 0.2M concentration. The measured and calculated parameters used to compare these matrices are good tools to rank and categorize matrix performances in MALDI-TOF MS measurements. The evaluation of matrices through crystal morphology, UV absorbance, extinction coefficient and hydrophobicity (logP) does not provide enough data to determine which is the best matrix. The determining factors seem to be the size of the molecule and its ability to stack over each other in the crystals. As shown by the MALDI-TOF MS data, the use of 3-HPA provided the highest S/N ratio and mass resolution. More studies are required to understand and correlate each parameter to the MALDI-TOF MS data.

机译：确认50％的DMF为每种MALDI基质提供足够的溶解度，高达0.2M浓度。用于比较这些矩阵的测量和计算的参数是在MALDI-TOF MS测量中等级和分类矩阵性能的好工具。通过晶体形态，UV吸光度，消光系数和疏水性（LOGP）的评估不提供足够的数据来确定哪个是最佳矩阵。确定因子似乎是分子的大小，并且其能够在晶体中堆叠堆叠。如MALDI-TOF MS数据所示，使用3-HPA提供最高的S / N比和质量分辨率。需要更多的研究来理解和将每个参数与MALDI-TOF MS数据相关联。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2010年||共1页
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Chue Hue Yang; Dickson Muoki Wambua; Norman H. L. Chiu;
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An In-Depth Parallel Study on Using Various MALDI Matrices for MicroRNA Analysis

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