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首页> 外文会议>American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics >Comparative Proteome Analyses Of Outer Membrane Proteins And Their Effect On Strain-level Discrimination Using Mass Spectrometry-based Proteomics Approach
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Comparative Proteome Analyses Of Outer Membrane Proteins And Their Effect On Strain-level Discrimination Using Mass Spectrometry-based Proteomics Approach

机译：外膜蛋白的比较蛋白质组分析及其对基于质谱的蛋白质组学方法的应变级别辨别的影响

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Recently the utility of mass spectrometry analysis to characterize and identify biological agents using a proteomic approach has been demonstrated. The present study sought to determine whether proteomics mass spectrometry could be used to distinguish between pathogenic and non-pathogenic strains of the same organism based on outer membrane proteins (OMPs). OMPs are often associated with virulence in gram-negative pathogens and could prove to be excellent model biomarkers for strain differentiation among bacteria. Therefore, OMPs were isolated from pathogenic and non-pathogenic strains of Yersinia pestis and Escherichia coli using ultracentrifugation and a sarkosyl extraction method followed by protein digestion and analysis using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The experimental MS/MS spectral data of bacterial peptides were searched using the SEQUEST algorithm against a constructed proteome database of microorganisms in order to determine the identity and number of unique peptides for each sample. Results showed that pathogenic strains could be distinguished from non-pathogenic strains for both organisms based on differences in OMP profiles. Virulent E. coli and Y. pestis expressed multiple OMPs that were not present in avirulent samples. In conclusion, proteomics mass spectrometry proved useful in discriminating between pathogenic and non-pathogenic strains of E. coli and Y. pestis and OMPs unique to pathogenic strains could prove to be useful biomarkers for strain differentiation.
机译：最近，已经证明了使用蛋白质组学方法表征和鉴定生物药物的质谱分析的效用。本研究试图确定蛋白质组学质谱质是否可用于区分基于外膜蛋白（OMP）的相同生物的病原和非致病菌株。 OMP通常与革兰阴性病原体的毒力有关，并且可以证明是细菌之间应变分化的优秀模型生物标志物。因此，使用超速离心和甲磺酰基提取方法和甲磺酰基提取方法与蛋白质瘟疫和大肠杆菌的致病和非致病菌株分离出OMP，然后使用液相色谱 - 质谱/质谱/质谱（LC-MS / MS）分析。使用续集算法对微生物的构建蛋白质组数据库进行搜索的实验MS / MS光谱数据，以确定每个样品的独特肽的身份和数量。结果表明，基于OMP型谱的差异，可以从两种生物的非致病菌株区分致病菌株。毒性的大肠杆菌和Y.Pestis表达了无毒样品中不存在的多个OMP。总之，蛋白质组学质谱证明，用于区分大肠杆菌和Y的致病和非致病菌菌株之间的鉴别诱导和致病菌菌株特异性的遗传和OMP可被证明是用于菌株分化的有用生物标志物。

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