Photo-Assisted Peptide Enrichment in Protein Complex Cross-Linking Analysis of a Transient Homodimeric Protein Using Mass Spectrometry

机译：蛋白质复杂交联分析瞬时聚二双聚体蛋白质的蛋白质复合肽富集的光辅助肽富集

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Homodimeric pilM protein from Pseudomonas aeruginosa 2192 (pilM) was investigated and the predicted pair of lysine side chains within 14A was cross-linked and enriched. The efficiency of the enrichment procedure was improved significantly with SSIN. The signal of MS/MS spectra was improved allowing for unambiguous sequence assignment. The method enables identification of low amounts of cross-linked peptides in an abundance of digested peptide mixtures for protein-protein interaction studies.

机译：研究了来自假单胞菌铜绿假单胞菌2192（PilM）的同型硅烷蛋白（pilm），并且在14A内预测的一对赖氨酸侧链被交联并富集。富集程序的效率显着提高了SSIN。改进了MS / MS光谱的信号，允许明确的序列分配。该方法能够鉴定蛋白质 - 蛋白质相互作用研究的丰富消化肽混合物中的少量交联肽。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2011年||共1页
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Funing Yan; Fa-yun Che; Edward Nieves; Andras Fiser; Louis M. Weiss; Ruth Hogue Angeletti;
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1. Photo-assisted peptide enrichment in protein complex cross-linking analysis of a model homodimeric protein using mass spectrometry. [J] . Yan F, Che FY, Nieves E, Proteomics . 2011,第20期

机译：使用质谱法对模型同型二聚体蛋白质进行蛋白质复合物交联分析时，光辅助肽富集。
2. Chemical cross-linking and high-performance Fourier transform ion cyclotron resonance mass spectrometry for protein interaction analysis: Application to a calmodulin/target peptide complex [J] . Kalkhof S, Ihling C, Mechtler K, Analytical chemistry . 2005,第2期

机译：化学交联和高性能傅里叶变换离子回旋共振质谱用于蛋白质相互作用分析：在钙调蛋白/靶标肽复合物中的应用
3. Analysis of protein-RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry [J] . Sharma Kundan, Hrle Ajla, Kramer Katharina, Methods: A Companion to Methods in Enzymology . 2015,第Null期

机译：紫外线诱导的交联和质谱分析CRISPR蛋白质和效应复合物中的蛋白质RNA相互作用
4. Photo-Assisted Peptide Enrichment in Protein Complex Cross-Linking Analysis of a Transient Homodimeric Protein Using Mass Spectrometry [C] . Funing Yan, Fa-yun Che, Edward Nieves, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：蛋白质复杂交联分析瞬时聚二双聚体蛋白质的蛋白质复合肽富集的光辅助肽富集
5. The analysis of proteins and peptides by mass spectrometry: From the whole cell proteome to small protein complexes. [D] . High, Anthony Andrew. 2003

机译：通过质谱分析蛋白质和多肽：从整个细胞蛋白质组到小型蛋白质复合物。
6. Photo-Assisted Peptide Enrichment in Protein Complex Cross-Linking Analysis of a Model Homodimeric Protein Using Mass Spectrometry [O] . Funing Yan, Fa-Yun Che, Edward Nieves, -1

机译：使用质谱法蛋白质复合蛋白质复合蛋白质复合蛋白质复合分析分析的照片辅助肽富集分析
7. Analysis of protein–RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry. [O] . Sharma, K., Hrle, A., Kramer, K., 2015

机译：通过UV诱导的交联和质谱分析CRIspR蛋白和效应复合物中的蛋白质-RNa相互作用。

Photo-Assisted Peptide Enrichment in Protein Complex Cross-Linking Analysis of a Transient Homodimeric Protein Using Mass Spectrometry

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