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首页> 外文会议>American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics >Preparation of Pure O-antigen Containing Saccharides from Francisella tularensis LPS for Vaccine Development Using Two-dimensional Liquid Chromatography
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Preparation of Pure O-antigen Containing Saccharides from Francisella tularensis LPS for Vaccine Development Using Two-dimensional Liquid Chromatography

机译：使用二维液相色谱法制备富含Francisella Turrensis LPS的富含O-抗原的糖果疫苗发育

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Francisella tularensis (Ft), a type of gram-negative bac terium, is best known as the cause of tularemia, an infectious disease in humans with low infectious dose and high mortality rate. Lipopolysaccharide (LPS) has been reported as a protective antigen (1). Three types of saccharides in Ft LPS that contain O -antigen (OAg) were described in our recent report, two consisting of tetrasa ccharide repeats attached to one of two core oligosaccharide variants, and one consisting of tetrasaccharide repeats without core (2). Since all three types of OAg-containing saccharides have potential to generate an immune response toward Ft, preparative chromatographic separation of glycans of defined length and composition is necessary to map epitopes targeted by protective anti-Ft LPS antibodies towards development of vaccines and immunotherapeutics for tularemia. This report details the successful preparation of pure LPS saccharides by a two dimensional chromatography method, as demonstrated using mass spectrometric analysis of the fractions.
机译：Francisella Tularensis（FT），一种革兰氏阴性Bac统一，是最为被称为Tularemia的原因，具有低传染剂量和高死亡率的人类的传染病。脂多糖（LPS）已被报告为保护性抗原（1）。在我们最近的报告中描述了含有o-antigen（OAG）的FT LP中的三种糖类，其中两种由连接到两个核糖寡糖变体中的一种组成的TetraA C结合重复，并且由没有核（2）的四糖重复组成。由于所有三种类型的含OAG-的糖具有潜在产生朝向尺的免疫应答，定义长度和组成的聚糖的制备色谱分离是必要的映射通过朝向疫苗和免疫治疗剂对兔热病的发展保护性抗呎LPS抗体靶向的表位。本报告详细说明了通过二维色谱法的成功制备纯LPS糖，如使用级分的质谱分析所证明的。

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