HPLC-ESI -MS/MS analysis of DNA adducts of cyclophosphamide in human peripheral blood lymphocytes and HapMap cells

机译：HPLC-ESI -MS / MS分析人外周血淋巴细胞和HAPMAP细胞中环磷酰胺的DNA加合物

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A quantitative capillary HPLC-ESI -MS/MS based methodology has been developed to accurately quantify CPA-induced G-NOR-OH monoadducts and G-NOR-G cross-links in HapMap human cell lines and peripheral blood lymphocytes. The new methodology was employed to investigate the formation and repair of CPA-induced DNA adducts in PBLs isolated from blood of ten healthy volunteers and in five human HapMap cell lines. Our results reveal significant inter-individual differences in the kinetics of G-NOR-G adduct formation and repair. Future studies will investigate the usefulness of DNA damage assessment in PBLs treated with reactive metabolites ex vivo as a predictive model of in vivo efficacy and toxicity in patients receiving CPA and other DNA alkylating agents.

机译：已经开发了定量毛细管HPLC-ESI -MS / MS MS的方法，以准确地量化HapMap人细胞系和外周血淋巴细胞中的CPA诱导的G-NOR-OH单涂层和G-NOR-G交联。使用新方法来研究从十个健康志愿者的血液中分离的PBLS中CPA诱导的DNA加合物的形成和修复，并在5人Hapmap细胞系中。我们的结果揭示了G-NOR-G加合物形成和修复动力学的显着间间差异。未来的研究将研究DNA损伤评估在用反应性代谢物离体处理的PBL治疗中的有用性作为接受CPA和其他DNA烷化剂的患者体内疗效和毒性的预测模型。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2013年||共1页
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Teshome B. Gherezghiher; Tanya J. Feldberg; LAurelle A. Johnson; Jatinder Lamba; Natalia Y. Tretyakova;
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HPLC-ESI -MS/MS analysis of DNA adducts of cyclophosphamide in human peripheral blood lymphocytes and HapMap cells

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