Femtosecond Laser-induced Ionization/Dissociation (fs-LID) Tandem Mass Spectrometry for the Enhanced Characterization of Low Charge State Protonated Phosphopeptide Ions

机译：Femtosecond激光诱导的电离/解离（FS-LID）串联质谱，用于增强低电荷态磷酸肽离子的表征

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fs-LID-MS/MS is viable for phosphopeptide analysis, providing: (1) comprehensive sequence information; (2) phosphorylation site identification. Positive mode fs-LID performs best for singly-protonated precursors: (1) fs-LID is complementary to CID and ETD; (2) fs-LID-MS/MS is suitable for MALDI-generated precursors. Negative mode fs-LID is best for multiply-deprotonated precursors: (1) negative precursors release electrons more easily; (2) preliminary data suggests negative mode may ultimately be more efficient than positive mode. Next: fs-LID-MS/MS on a MALDI-equipped 2-D quadrupole ion trap: (1) greater ion/laser overlap; (2) greater sensitivity; (3) faster analysis times; (4) permits combined strategies: on-plate enrichment and fs-LID-MS/MS.

机译：FS-LID-MS / MS可用于磷酸肽分析，提供：（1）综合序列信息; （2）磷酸化位点鉴定。正模式FS-LID为单个质子化前体执行最佳：（1）FS-LID与CID和ETD互补; （2）FS-LID-MS / MS适用于MALDI产生的前体。负模式Fs-led最适合乘法去质子化前体：（1）负前体更容易释放电子; （2）初步数据表明负模式最终可能比正模式更有效。下一页：配备MALDI的2-D Quadrupole离子阱上的FS-LID-MS / MS：（1）更大的离子/激光重叠; （2）更大的敏感性; （3）更快的分析时间; （4）允许合并策略：板材浓缩和FS-LID-MS / MS。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2010年||共1页
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Scott A. Smith; Christine L. Kalcic; Kyle A. Safran; Paul M. Stemmer; Marcos Dantus; Gavin E. Reid;
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1. Enhanced Characterization of Singly Protonated Phosphopeptide Ions by Femtosecond Laser-induced Ionization/Dissociation Tandem Mass Spectrometry (fs-LID-MS/MS) [J] . Smith S.A., Kalcic C.L., Safran K.A., Journal of the American Society for Mass Spectrometry . 2010,第12期

机译：飞秒激光诱导的电离/解离串联质谱（fs-LID-MS / MS）增强了对单质子化磷酸肽离子的表征
2. Femtosecond laser-induced ionization/dissociation tandem mass spectrometry (fsLID-MS/MS) of deprotonated phosphopeptide anions [J] . Scott A. Smith, Christine L. Kalcic, Li Cui, Rapid Communications in Mass Spectrometry: RCM . 2013,第24期

机译：飞秒激光诱导的去质子化磷酸肽阴离子的电离/解离串联质谱（fsLID-MS / MS）
3. Femtosecond laser-induced dissociation (fs-LID) as an activation method in mass spectrometry [J] . Neidel Christian, Kuehn Andreas, Schulz Claus Peter, Chemical Physics: A Journal Devoted to Experimental and Theoretical Research Involving Problems of Both a Chemical and Physical Nature . 2018,第期

机译：Femtosecond激光诱导的解离（FS-LID）作为质谱法的激活方法
4. Femtosecond Laser-induced Ionization/Dissociation (fs-LID) Tandem Mass Spectrometry for the Enhanced Characterization of Low Charge State Protonated Phosphopeptide Ions [C] . Scott A. Smith, Christine L. Kalcic, Kyle A. Safran, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：Femtosecond激光诱导的电离/解离（FS-LID）串联质谱法，用于增强低电荷状态质子化磷酸肽离子的表征
5. Dissociation of protonated peptides: Structure elucidation, energetics and mechanisms by tandem mass spectrometry. [D] . Zhong, Wenqing. 1999

机译：质子化肽的解离：串联质谱的结构阐明，能量学和机理。
6. Revisiting Fragmentation Reactions of Protonated α-Amino Acids by High-Resolution Electrospray Ionization Tandem Mass Spectrometry with Collision-Induced Dissociation [O] . Pengwei Zhang, Wan Chan, Irene L. Ang, -1

机译：碰撞诱导解离的高分辨率电喷雾串联质谱研究质子化α-氨基酸的裂解反应
7. Enhanced Characterization of Singly Protonated Phosphopeptide Ions by Femtosecond Laser-induced Ionization/Dissociation Tandem Mass Spectrometry (fs-LID-MS/MS) [O] . Smith Scott A., Kalcic Christine L., Safran Kyle A., 2010

机译：飞秒激光诱导的电离/解离串联质谱（fs-LID-MS / MS）增强了对单质子化磷酸肽离子的表征
8. Capillary Column Gas Chromatography, Resonance Enhanced Multiphoton Ionization, Time-of-Flight Mass Spectrometry Laser-Induced Fluorescence, Flame Ionization Detection System for the Determination of Polynuclear Aromatic Compounds [R] . Dobson, R. L. M. 1986

机译：毛细管柱气相色谱，共振增强多光子电离，飞行时间质谱激光诱导荧光，火焰离子化检测系统测定多核芳香族化合物

Femtosecond Laser-induced Ionization/Dissociation (fs-LID) Tandem Mass Spectrometry for the Enhanced Characterization of Low Charge State Protonated Phosphopeptide Ions

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