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A Scalable Process for Helper-Dependent Adenoviral Vector Production Using PEI-Derived Transfection Strategy in Suspension Culture

机译：使用Pei衍生的转染策略在悬浮培养中的辅助依赖性腺病毒载体产生的可扩展方法

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Helper-dependent adenoviral vector has demonstrated a high therapeutic potential; however, the poor efficiency of the actual production process hampers further large-scale clinical evaluation. In this work, we evaluated the adenofection protocol, an efficient polyethylenimine transfection strategy, for HDV production. Optimized conditions of PEI-adenofection were used to generate HDV at 3-L biore-actor scale. This process strategy realized in suspension reduced process duration and therefore ensured production of high-quality vector stock.

机译：辅助依赖性腺病毒载体展示了高治疗潜力;然而，实际生产过程的效率差妨碍了进一步大规模的临床评价。在这项工作中，我们评估了腺激素方案，一种高效的聚对乙基转染策略，用于HDV生产。 Pei-adenofection的优化条件用于在3-L生物actor刻度产生HDV。这种过程策略在暂停中实现了减少工艺持续时间，因此确保了高质量的矢量股票的生产。

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《European Society of Animal Cell Technology》|2010年||共6页
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作者
E. Dormond; A. Meneses-Acosta; D. Jacob; Y. Durocher; M. Perrier; and A. Kamen;
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中图分类动物细胞学;
关键词
Helper-dependent adenoviral vector; HEK 293; PEI-transfection; Suspension cells; Bioreactor;

机译：辅助依赖性腺病毒载体;HEK 293;PEI转染;悬浮细胞;生物反应器;

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专利

1. Development of a scalable process for high-yieldlentiviral vector production by transienttransfection of HEK293 suspension cultures [J] . Sven Ansorge, Stephane Lanthier, Julia Transfiguracion, The journal of gene medicine . 2009,第10期

机译：通过瞬时转染HEK293悬浮培养物生产高产力慢病毒载体的可扩展方法的开发
2. An Efficient and Scalable Process for Helper-Dependent Adenoviral Vector Production Using Polyethylenimine-Adenofection [J] . Dormond E, Mencses-Acosta A, Jacob D, Biotechnology and Bioengineering . 2009,第3期

机译：一个高效和可扩展的过程，使用聚乙烯亚胺腺苷感染的辅助依赖型腺病毒载体生产。
3. Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification [J] . Segura MM, Garnier A, Durocher Y, Biotechnology and Bioengineering . 2007,第4期

机译：通过悬浮培养物的大规模瞬时转染和亲和层析纯化产生慢病毒载体
4. A Scalable Process for Helper-Dependent Adenoviral Vector Production Using PEI-Derived Transfection Strategy in Suspension Culture [C] . E. Dormond, A. Meneses-Acosta, D. Jacob, European Society of Animal Cell Technology . 2010

机译：使用Pei衍生的转染策略在悬浮培养中的辅助依赖性腺病毒载体产生的可扩展方法
5. Development of a scalable process for lentiviral vector mass production by transient transfection. [D] . Ansorge, Sven. 2010

机译：通过瞬时转染开发可大规模生产慢病毒载体的方法。
6. Large-Scale Production of High-Quality Helper-Dependent Adenoviral Vectors Using Adherent Cells in Cell Factories [O] . Masataka Suzuki, Racel Cela, Christian Clarke, -1

机译：使用细胞工厂中的贴壁细胞大规模生产高质量的依赖助手的腺病毒载体
7. Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification [O] . Segura, Maria Mercedes, Garnier, Alain, Durocher, Yves, 2007

机译：通过悬浮培养物的大规模瞬时转染和亲和层析纯化产生慢病毒载体

A Scalable Process for Helper-Dependent Adenoviral Vector Production Using PEI-Derived Transfection Strategy in Suspension Culture

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