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首页> 外文会议>Annual and International Meeting of the Japanese Association for Animal Cell Technology >Dendrimer-Immobilized Culture Surface as a Tool to Promote Aggregate Formation of Anchorage-Dependent Cells
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Dendrimer-Immobilized Culture Surface as a Tool to Promote Aggregate Formation of Anchorage-Dependent Cells

机译：Dendimer-固定化培养物作为促进依赖依赖性细胞的聚集体形成的工具

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In cultures of anchorage-dependent cells, the changes in cell morphology can cause variations in cell behaviors, and culture surface design is often employed to regulate the cell morphology. In our previous study, we designed the transporter-mediatedanchoring by displaying D-glucose on a glass surface, and reported that the shape of rabbit chondrocytes and their adhesion were closely related to the density of D-glucose displayed on the surface. In the cultures with changed ratios of D- and L-glucose, it was found that 100% D-glucose-displayed surface provided a population of round-shaped cells with the promoted extracellular matrix (ECM) production. In the extended work with human mammary epithelial cells, the varied densities of D-glucose displayed were prepared by using starburst polyamidoamine dendrimer, which is a promising template for ligand display at terminals of dendric structure.
机译：在依赖于依赖依赖细胞的培养中，细胞形态的变化会导致细胞行为的变化，并且通常采用培养表面设计来调节细胞形态。在我们以前的研究中，我们通过在玻璃表面上显示D-葡萄糖设计了转运膜介导的，并且报道了兔软骨细胞的形状及其粘附与表面上显示的D-葡萄糖的密度密度密度。在具有D-和L-葡萄糖的变化比率的培养物中，发现100％D-葡萄糖显示表面提供圆形细胞群，具有促进的细胞外基质（ECM）生产。在与人乳腺上皮细胞的延长工作中，通过使用Sarburst Polyamidoamine Dendrimer制备的D-葡萄糖的不同密度，这是在任命结构的端子处的配体显示器的有希望的模板。

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