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首页> 外文会议>Annual and International Meeting of the Japanese Association for Animal Cell Technology >Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production
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Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production

机译：抗体纯化后使用溶液增强抗体产生：在抗体生产中的应用

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Monoclonal antibodies are essential in fields of diagnostics and medicine. The market for antibody drugs is growing every year by 20% but antibody production has some risks including limited supply and high costs because antibodies are produced by mammalian cells such as hybridomas and Chinese hamster ovary (CHO) cells. These cells are cultured in expensive serum-free media containing growth factors in order to maintain cellular functions. In particular, transferrin and insulin are essential for theproduction of antibodies; transferrin functions as an iron transporter and a mitogen, while insulin improves glucose uptake. However, as these growth factors remain after cell culture, the used culture media are typically discarded. Riese et al. have reported that used culture medium was effective for the production of mouse monoclonal antibodies when it was mixed with additional amino acids and glucose, after purification by microfiltration and ultra filtration.
机译：单克隆抗体在诊断和医学领域是必不可少的。抗体药物的市场每年增长20％，但抗体产量具有一些风险，包括供应有限和高成本，因为抗体由哺乳动物和中国仓鼠卵巢（CHO）细胞等哺乳动物细胞产生。这些细胞在含有生长因子的昂贵无血清培养基中培养，以保持细胞功能。特别地，转铁蛋白和胰岛素对于抗体的生产是必不可少的;转铁蛋白用作铁转运蛋白和丝分裂原，而胰岛素改善葡萄糖摄取。然而，由于细胞培养后这些生长因子留下，通常丢弃使用的培养基。 Riese等。据报道，当通过微滤和超滤后纯化后，使用培养基在与另外的氨基酸和葡萄糖混合时，培养培养基的生产是有效的。

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