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首页> 外文会议>International Gastric Cancer Congress >Analysis of cDNA Microarray for Identification of Genes Up-regulated by Demethylating Agents in Gastric Cancer Cell Lines
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Analysis of cDNA Microarray for Identification of Genes Up-regulated by Demethylating Agents in Gastric Cancer Cell Lines

机译：CDNA微阵列分析CDNA微阵列，以鉴定胃癌细胞中去甲基化试剂上调基因的鉴定

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In this study, we employed cDNA microarray analysis to identify genes up-regurated by Procain (PCA) treatment in gastric cancer cell line and compared with genes up-regurated by DAC treatment. We surveyed up-regulated genes to discover aberrantly methylated gene and determined the methylation status of 5'CpG islands by bisufite DNA sequencing.As a result of classification as for function, genes related to "cell cycle" and "apoptosis" were up-regulated by both PCA and by DAC treatment. However, there was a significant difference in up-regulated genes between PCA and DAC treatment, suggesting difference of the growth inhibitory mechanism. Using bisufite DNA sequencing, a gene related to apoptosis, diva was found to be transcriptionally repressed by promoter hypermethylation and this gene might be related to gastric carcinogenesis.
机译：在该研究中，我们使用cDNA微阵列分析，以鉴定胃癌细胞系中ProCain（PCA）治疗的基因，并与DAC治疗悬垂的基因相比。我们调查了上调基因，以发现异常甲基化基因，并通过双富矿DNA测序确定5'CPG岛的甲基化状态。作为函数的分类结果，上调了与“细胞周期”和“细胞凋亡”相关的基因通过PCA和DAC治疗。然而，PCA和DAC治疗之间的上调基因存在显着差异，表明生长抑制机制的差异。使用Bisufite DNA测序，发现与细胞凋亡相关的基因，发现DIVA通过启动子超甲基化转录压抑，该基因可能与胃癌发生有关。

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