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首页> 外文会议>ASME Bioengineering Conference >SUPERCRITICAL CARBON DIOXIDE STERILIZATION MINIMALLY AFFECTS HUMAN ALLOGRAFT SKIN MORPHOLOGY AND BIOMECHANICS
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SUPERCRITICAL CARBON DIOXIDE STERILIZATION MINIMALLY AFFECTS HUMAN ALLOGRAFT SKIN MORPHOLOGY AND BIOMECHANICS

机译：超临界二氧化碳灭菌微量地影响人同种异体联系皮肤形态和生物力学

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Skin-based products are currently used to treat numerous acute and chronic injuries. Allograft skin typically used for burn treatment is recovered aseptically and either cryopreserved or provided fresh shortly after procurement in order to maintain viability for engraftment. Such skin is also further processed in a variety of ways, including decellularization and lyophilization, to create biologic dressings and skin substitutes. Although allograft skin is vigorously screened and disinfected to prevent pathogen transmission it is seldom terminally sterilized, which may be of concern when it is used for patients who are immunocompromised. Thus, the overall goal of this work is to develop a sterile, skin-based biomaterial for potential use as a matrix for tissue regeneration. A new sterilization technology was investigated in this study because gamma irradiation of skin is largely avoided due to collagen crosslinking that can cause stiffening (1). Supercritical carbon dioxide (SC-CO_2) is potentially advantageous for soft tissue sterilization given its bacterial and viral inactivation capabilities. Also, SC-CO_2 has been shown to decellularize cardiovascular tissue without the need for surfactants (2). However, data on SC-CO_2 sterilization of skin remains limited. The objective of this study was to characterize the effects of SC-CO_2 sterilization on the morphology, sterilization, and biomechanical properties of allograft skin. Our hypotheses were that SC-CO_2 would 1) decellularize the skin, 2) eradicate any existing bioburden, and 3) minimally affect the failure biomechanics.
机译：基于皮肤的产品目前用于治疗多种急性和慢性损伤。通常用于烧伤治疗异体皮无菌回收，无论是冷冻保存或以维持生存为移植后的采购提供新鲜不久。这样的皮肤也进一步以各种方式，包括脱细胞和冻干，打造生物敷料和皮肤替代物进行处理。虽然异体皮大力筛选，消毒，防止病原传播它很少被最终灭菌，当它被用来谁是免疫力低下的病人可能是关注的。因此，这项工作的总体目标是开发用于组织再生基质的潜在应用无菌的，基于皮肤的生物材料。一种新的灭菌技术在本研究中进行了研究，因为皮肤的γ照射在很大程度上避免由于交联的胶原蛋白，可导致加强（1）。超临界二氧化碳（SC-CO_2）可以鉴于其细菌和病毒失活的能力的软组织灭菌潜在有利的。此外，SC-CO_2已经显示出心血管脱细胞组织而不需要表面活性剂（2）。然而，对皮肤的遗体SC-CO_2杀菌数据限制。这项研究的目的是表征的形态，杀菌和异体皮的生物力学性能SC-CO_2杀菌的效果。我们的假设是SC-CO_2会1）的脱细胞化皮肤，2）消除任何现有的生物负荷，以及3）影响最小的失败生物力学。

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