A MICROFLUIDIC DEVICE FOR AUTOMATED ELECTROPHYSIOLOGICAL MEASUREMENTS ON XENOPUS OOCYTES UNDER ZERO GRAVITY

机译：用于零重力下的卵宫卵母细胞的自动电生理测量的微流体装置

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A non-invasive electrophysiology system for performing rapid measurements on Xenopus laevis oocytes under varying gravity levels is presented. Our novel approach makes the need for inserting microelectrodes into the cytosol unnecessary. Immobilization of the oocyte on top of an orifice is achieved using a slight air overpressure to ensure that the cell is kept in a stable condition even under varying gravity levels. In our present studies, we overexpressed the epithelial sodium channel (ENaC) and the sodium/phosphate cotransporter (NaPi-IIb). Results obtained under laboratory conditions as well as under microgravity and hypergravity demonstrate the system's robustness and give first indications of ENaC's susceptibility to changes in gravity.

机译：提出了一种用于在不同重力水平下对Xenopus Laevis卵母细胞进行快速测量的非侵入式电生理系统。我们的新方法使得需要将微电极插入到不需要的细胞溶胶中。使用轻微的空气过压来实现将卵母细胞固定在孔口顶部，以确保即使在不同的重力水平下也能够保持稳定状态的细胞。在我们目前的研究中，我们过表达了上皮钠通道（ENAC）和钠/磷酸钠/磷酸钠（NaPi-IIB）。在实验室条件下获得的结果以及微匍匐和超级争论展示了系统的稳健性，并提供了ENAC对重力变化的敏感性的第一个指示。

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《International Conference on Miniaturized Systems for Chemistry and Life Sciences》|2011年||共3页
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D.S. Schaffhauser; O. Andrini; C. Ghezzi; M. Schaffner; I.C. Forster; A. Franco-Obregón; M. Egli; P.S. Dittrich;
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中图分类 O6-532;
关键词
electrophysiology; non-invasive; Xenopus laevis; epithelial sodium channel; sodium phosphate cotransporter; gravity;

机译：电生理;非侵入性;Xenopus laevis;上皮钠通道;磷酸钠cotroanger;重力;

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2. Microfluidic platform for electrophysiological studies on Xenopus laevis oocytes under varying gravity levels [J] . Daniel F. Schaffhauser, Olga Andrini, Chiara Ghezzi Lab on a chip . 2011,第20期

机译：在不同重力水平下非洲爪蟾卵母细胞电生理研究的微流体平台
3. Integrated microsystem for non-invasive electrophysiological measurements on Xenopus oocytes [J] . Dahan E, Bizeb V, Lehnert T, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2007,第12期

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5. A MICROFLUIDIC DEVICE FOR AUTOMATED ELECTROPHYSIOLOGICAL MEASUREMENTS ON XENOPUS OOCYTES UNDER ZERO GRAVITY [C] . D.S. Schaffhauser, O. Andrini, C. Ghezzi, International Conference on Miniaturized Systems for Chemistry and Life Sciences . 2011

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8. Microfluidic platform for electrophysiological studies on Xenopus laevis oocytes under varying gravity levels [O] . Schaffhauser, D F, Andrini, O, Ghezzi, C, 2011

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A MICROFLUIDIC DEVICE FOR AUTOMATED ELECTROPHYSIOLOGICAL MEASUREMENTS ON XENOPUS OOCYTES UNDER ZERO GRAVITY

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