A DISPOSABLE DNA AMPLIFICATION PLATFORM FOR THE DETECTION OF CLOSTRIDIUM DIFFICILE INFECTED STOOL SPECIMENS

机译：用于检测梭菌差异感染粪便标本的一次性DNA扩增平台

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We present a disposable DNA amplification platform for helicase-dependent isothermal amplification (HDA) designed for the detection of Clostridium difficile in human stool specimens. This platform consists of a low-cost, thermoplastic reaction chip, a pair of commercially available toe warmers commonly used by skiers as a chemical heater, and styrofoam cups as insulators. The platform is capable of maintaining the optimum temperature for HDA at 65°C ± 2°C for more than 55 min. When compared to reactions performed using thermocycler, this system has a comparable DNA detection limit (1.25x10~(-2) pg) using cheap, readily available materials with no need for electricity.

机译：我们介绍了一种用于螺旋酶依赖性等温扩增（HDA）的一次性DNA扩增平台，用于检测人粪便标本中的梭菌差异。该平台由低成本，热塑性反应芯片组成，一对商业上可获得的脚趾加热器，通常用于滑雪者作为化学加热器，以及聚苯乙烯泡沫塑料杯作为绝缘体。该平台能够在65℃±2℃下保持HDA的最佳温度超过55分钟。与使用热循环仪进行的反应相比，该系统具有可比较的DNA检测限（1.25x10〜（-2）pg）使用便宜的，易于获得的材料，无需电力。

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《International Conference on Miniaturized Systems for Chemistry and Life Sciences》|2011年||共3页
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作者
S. Huang; J. Do; M. Mahalanabis; A. Fan; L. Jepeal; S. Singh; C. Klapperich;
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中图分类 O6-532;
关键词
Helicase-dependent amplification (HDA); Disposable; Clostridium difficile; Stool; Thermoplastic;

机译：依赖螺旋酶依赖性扩增（HDA）;一次性;艰难速度;凳子;热塑性塑料;

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专利

1. Application of isothermal helicase-dependent amplification with a disposable detection device in a simple sensitive stool test for toxigenic Clostridium difficile. [J] . Chow WH, McCloskey C, Tong Y, The Journal of molecular diagnostics: JMD . 2008,第5期

机译：等温解旋酶依赖性扩增与一次性检测装置在产毒性艰难梭菌的简单敏感粪便检测中的应用。
2. Evaluation of Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile Assays for Direct Detection of Toxigenic Clostridium difficile in Stool Specimens [J] . Annals of laboratory medicine. . 2016,第2期

机译：雅培m2000的Xpert艰难梭菌，BD MAX Cdiff，IMDx艰难梭菌和艰难梭菌Illumigene艰难梭菌检测法在粪便标本中直接检测产艰难梭菌的评估
3. Portrait Toxigenic Clostridium difficile assay, an isothermal amplification assay detects toxigenic C. difficile in clinical stool specimens [J] . DenysG.A. Expert Review of Molecular Diagnostics . 2014,第1期

机译：人像毒性艰难梭菌测定法，等温扩增测定法可检测临床粪便标本中的艰难梭菌
4. A DISPOSABLE DNA AMPLIFICATION PLATFORM FOR THE DETECTION OF CLOSTRIDIUM DIFFICILE INFECTED STOOL SPECIMENS [C] . S. Huang, J. Do, M. Mahalanabis, International Conference on Miniaturized Systems for Chemistry and Life Sciences . 2011

机译：用于检测梭菌差异感染粪便标本的一次性DNA扩增平台
5. Plasmid DNA as a Platform for the Development of Prophylactic and Therapeutic Vaccines Against Clostridium difficile in Aging Models of Disease [D] . Ramamurthi, Arjun 2017

机译：质粒DNA作为在疾病老龄化模型中开发针对艰难梭菌的预防和治疗疫苗的平台
6. Application of Isothermal Helicase-Dependent Amplification with a Disposable Detection Device in a Simple Sensitive Stool Test for Toxigenic Clostridium difficile [O] . Wing Huen A. Chow, Cindy McCloskey, Yanhong Tong, 2008

机译：等温解旋酶依赖性扩增与一次性检测装置在产毒艰难梭菌的简单灵敏凳测试中的应用
7. Application of Isothermal Helicase-Dependent Amplification with a Disposable Detection Device in a Simple Sensitive Stool Test for Toxigenic Clostridium difficile [O] . Chow, Wing Huen A., McCloskey, Cindy, Tong, Yanhong, 2008

机译：等温解旋酶依赖性扩增与一次性检测装置在产毒艰难梭菌的简单灵敏凳测试中的应用

A DISPOSABLE DNA AMPLIFICATION PLATFORM FOR THE DETECTION OF CLOSTRIDIUM DIFFICILE INFECTED STOOL SPECIMENS

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