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首页> 外文会议>International Conference on Miniaturized Systems for Chemistry and Life Sciences >MICROFLUIDIC CHROMATIN IMMUNOPRECIPITATION ASSAY FOR HISTONE MODIFICATION ANALYSIS BASED ON 50 CELLS
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MICROFLUIDIC CHROMATIN IMMUNOPRECIPITATION ASSAY FOR HISTONE MODIFICATION ANALYSIS BASED ON 50 CELLS

机译：基于50个细胞的微流体染色质免疫沉淀法测定组蛋白修饰分析

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Histone modifications are important epigenetic mechanisms involved in eukaryotic gene regulation. Chromatin immunoprecipitation (ChIP) assay serves as the prime technique to characterize the genomic locations associated with histone modifications. However, traditional tube-based ChIP assays rely on large numbers of cells as well as laborious and time-consuming procedures. Here we demonstrate a novel microfluidics-based native ChIP assay which dramatically reduces the required cell number and the assay time by conducting cell collection, cell lysis, chromatin fragmentation, immunoprecipitation, and washing on a microchip. Coupled with real-time PCR, our assay permits the analysis of histone modifications from as few as -50 cells within 8.5 h.
机译：组蛋白修饰是涉及真核基因调控的重要表观遗传机制。染色质免疫沉淀（芯片）测定用作表征与组蛋白修饰相关的基因组位置的素化技术。然而，传统的基于管的芯片测定依赖于大量的细胞以及费力和耗时的程序。在这里，我们证明了一种新的基于微流体的天然芯片测定，通过导电细胞收集，细胞裂解，染色质片段化，免疫沉淀和在微芯片上洗涤，显着降低所需的细胞数和测定时间。再加上实时PCR，我们的测定允许在8.5小时内从少量-50个细胞分析组蛋白修饰。

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