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Biochar addition to soil changed microbial community structure and decreased microbial biomass carbon and net inorganic nitrogen mineralised

机译:生物炭加入土壤发生变化微生物群落结构和微生物生物质碳和净无机氮矿化

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Biochar is a recalcitrant carbon rich soil amendment proposed to improve soil fertility. We investigated changes in soil microbial biomass, community structure and function and net inorganic nitrogen changed resulting from its addition. We homogenously incorporated bibchar at rates of 0, 5, and 25 t/ha into a coarse textured sand in a glasshouse trial. Three nitrogen treatments were added: organic nitrogen (a pelleted pig manure and wheat straw compost), inorganic nitrogen (33 mg kg~(-1) of N as NH_4NO_3 at 3 and 6 weeks), and a control treatment. Wheat {Triticum aestivum cv. Calingiri) was grown for 70 days, after which samples were taken. Microbial biomass carbon (MBC) decreased with biochar addition (P=0.05). Microbial carbon to nitrogen ratio and microbial community structure changed significantly with biochar addition at 25 t/ha but not at 5 t/ha. The decrease in microbial C: N ratio (from 8:1 to 5:1) suggests a shift to a more bacteria dominant community. Net nitrogen mineralisation decreasedfrom an average of 11 mg N/kg dry soil after 10 weeks of incubation without biochar to 1 mg N/kg dry soil with addition at 25 t/ha. Sorption of nitrogen to biochar at these rates could not explain the result.
机译:Biochar是一种顽固的碳富含土壤修正案,提出改善土壤肥力。我们调查了土壤微生物生物量,群落结构和功能的变化,净无机氮因其添加而改变。我们将Bibchar融合在0,5和25吨/公顷的速率下,进入粗糙的纹理砂中。加入三种氮气处理:有机氮(沉淀的猪粪和小麦秸秆堆肥),无机氮(33mg kg〜(-1)n为nh_4nO_3,3和6周)和对照处理。小麦{triticum aestivum cv。 Calingiri)已经生长70天,之后取样。微生物生物质碳(MBC)随生物炭加入减少(P = 0.05)。微生物碳与氮含量和微生物群落结构在25t / ha的25t / ha下加入显着变化,但不为5t / ha。微生物C:n比(从8:1至5:1)的降低表明转变为更多细菌的主导群落。净氮矿化在孵育10周后平均每根平均孵育11mg n / kg干燥土壤,在25t / ha下加入1mg n / kg干燥土壤。这些速率下氮对生物炭的吸附无法解释结果。

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