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首页> 外文会议>Congress of the International Society for Mushroom Science >Possible Involvement of Reactive Oxygen Species in Ectomycorrhizal Association between Rhizopogon roseolus and Pinus thunbergii
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Possible Involvement of Reactive Oxygen Species in Ectomycorrhizal Association between Rhizopogon roseolus and Pinus thunbergii

机译：在Rhizogon Roseolus和Pinus thunbergii之间的Ecectomycorrhizal关联中可能参与反应性氧

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Possible involvement of reactive oxygen species in ectomycorrhizal association between Rhizopogon roseolus and Pinus thunbergii was microscopically investigated after nitroblue tetrazolium (NBT) staining. Roots of P. thunbergii were aseptically infected with R. roseolus mycelia by using the Petri dish technique. Initial mycorrhizal formation could be observed from 2 to 4 weeks after inoculation. NBTtreated lateral root tips were then observed under a light microscope. Blue formazan depositions indicating O2~ accumulation were detected mainly in hyphal cells in contact with the root surface. Observations of a transverse section of the root revealed blue formazan depositions at the plasma membranes of epidermal cells to which fungal hyphae were adhesively attached. In the non-inoculated P. thunbergii roots, formazan depositions were observed in root hair cells, but not in epidermal cells. Dichotomous mycorrhizas and extraradical mycelia were clearly observed from 4 to 8 weeks after inoculation. A section of NBT-treated mycorrhizas showed that the root tissue was surrounded by the fungal mantle sheath in which highly intensive reaction with NBT was observed. Reactive formazan complexes were apparent in Hartig net hyphae between root epidermal and cortical cells. After 16 weeks following inoculation, morphology of mycorrhizas became variable, viz., initial, dichotomous and browned mycorrhizas. The browned mycorrhizas were characterized by wrinkled surfaces and sparse extraradical mycelia. A section of NBT-treated browned mycorrhizas showed slight formazon depositions only in a part of extraradical mycelia. These results suggest that O2-generation in both fungi and plants is involved in the early mycorrhizal association between R. roseolus and P. thunbergii.
机译：在Nitroblue四唑（NBT）染色后，在Rhizogon Roseolus和Pinus thunbergii之间的EctOmycorrhizal结合中可能涉及反应性氧物种在硝基唑（NBT）染色之后是显微诊断的。通过使用Petri Dish技术无菌地无菌地感染R. Roseolus菌丝体。在接种后2至4周可以观察到初始菌根形成。然后在光学显微镜下观察NBTRATEATED横向根尖。指示O2〜积聚的蓝色甲卓沉积主要在与根表面接触的亚膦细胞中检测。对根部的横截面的观察显示在表皮细胞的血浆膜上的蓝色甲唑沉积，粘性菌丝粘地附着。在非接种的P.Thunbergii根中，在根毛细胞中观察到甲藻沉积，但不能在表皮细胞中观察。在接种后4至8周清楚地观察到二分的菌根和肌菌体。 NBT处理的菌根的一部分显示，根组织被真菌搭腔鞘包围，其中观察到与NBT的高度密集反应。在根表皮和皮质细胞之间的Hartig Net Hyphae中，反应性甲卓复合物在Hartig Net Hyphae中显而易见。接种后16周后，菌根的形态变为变量，敏捷，初始，二分法和褐色的菌根。褐色的菌根，其特征是由皱纹表面和稀疏的肌肉菌丝体。 NBT处理的褐皮菌疹的一部分仅在菌丝体的一部分中显示出轻微的甲型沉积。这些结果表明，真菌和植物中的O2发电都参与了R. Roseolus和P.Thunbergii之间的早期菌根关联。

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