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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Possible Involvement of Reactive Oxygen Species in Ectomycorrhizal Association between Rhizopogon roseolus and Pinus thunbergii
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Possible Involvement of Reactive Oxygen Species in Ectomycorrhizal Association between Rhizopogon roseolus and Pinus thunbergii

机译：活性氧参与根瘤菌与黑松松柏菌根缔合的可能

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摘要

Possible involvement of reactive oxygen species in ectomycorrhizal association between Rhizopogon roseolus and Pinus thunbergii was microscopically investigated after nitroblue tetrazolium (NBT) staining. Roots of P. thunbergii were aseptically infected with R. roseolus mycelia by using the Petri dish technique. Initial mycorrhizal formation could be observed from 2 to 4 weeks after inoculation. NBTtreated lateral root tips were then observed under a light microscope. Blue formazan depositions indicating O2~ accumulation were detected mainly in hyphal cells in contact with the root surface. Observations of a transverse section of the root revealed blue formazan depositions at the plasma membranes of epidermal cells to which fungal hyphae were adhesively attached. In the non-inoculated P. thunbergii roots, formazan depositions were observed in root hair cells, but not in epidermal cells. Dichotomous mycorrhizas and extraradical mycelia were clearly observed from 4 to 8 weeks after inoculation. A section of NBT-treated mycorrhizas showed that the root tissue was surrounded by the fungal mantle sheath in which highly intensive reaction with NBT was observed. Reactive formazan complexes were apparent in Hartig net hyphae between root epidermal and cortical cells. After 16 weeks following inoculation, morphology of mycorrhizas became variable, viz., initial, dichotomous and browned mycorrhizas. The browned mycorrhizas were characterized by wrinkled surfaces and sparse extraradical mycelia. A section of NBT-treated browned mycorrhizas showed slight formazon depositions only in a part of extraradical mycelia. These results suggest that O2-generation in both fungi and plants is involved in the early mycorrhizal association between R. roseolus and P. thunbergii.
机译：硝基蓝四唑（NBT）染色后，通过显微镜研究了活性氧可能参与了根瘤菌和黑松之间的菌根结合。通过使用陪替氏培养皿技术，用红玫瑰菌菌丝体无菌感染黑松柏根。接种后2-4周可观察到最初的菌根形成。然后在光学显微镜下观察经NBT处理的侧根尖端。主要在与根表面接触的菌丝细胞中检测到表明O2〜积累的蓝色甲maz沉积。观察到根部的横截面，发现在表皮细胞的质膜上附着有真菌菌丝的蓝色甲maz沉积。在未接种的雷氏假单胞菌根中，在根毛细胞中观察到甲maz沉积，但在表皮细胞中未观察到甲for沉积。接种后4至8周清楚地观察到二歧菌根和根外菌丝。经NBT处理的菌根的一部分显示，根部组织被真菌套膜包围，其中观察到与NBT的高度强烈反应。根表皮和皮层细胞之间的Hartig净菌丝中有明显的反应性甲maz络合物。接种后16周，菌根的形态变得可变，即初始，二分和褐色菌根。褐变的菌根的特征是表面起皱和根部菌丝稀疏。 NBT处理过的褐色菌根的一部分仅在部分根外菌丝体中显示出少量的甲maz沉积。这些结果表明，真菌和植物中的O2生成都参与了迷迭香玫瑰和黑僵菌之间的早期菌根结合。

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