Genetic Transformation of V. vinifera - Two Alternative Systems under Evaluation

机译：V.Vinifera - 两种替代系统评估的遗传转化

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'Trincadeira' and 'Aragonez' are two of the most important Vitis vinifera L. cultivars used for red wine production in Portugal. Aiming to obtain a reproducible system for plant genetic transformation, the techniques of particle bombardment and transformation mediated by Agrobacterium tumefaciens were tested. In both systems putative transformed embryos were selected by continued proliferation on an in vitro medium containing 40 mg/L of kanamycin. One week after co-culture the embryo-genie calli produced the first somatic embryos and four months later plantlets were regenerated from A. tumefaciens transformed calli. Results suggest this method as the most appropriate to transform embryogénie calli of both cultivars.

机译：'Trincadeira'和'Aragonez'是葡萄牙中用于红葡萄酒的最重要的葡萄藻氏族栽培品种。旨在获得用于植物遗传转化的可重复性系统，测试了Tumefaciens介导的粒子轰击和转化的技术。在两种系统中，通过在含有40mg / L的卡那霉素的体外培养基上继续增殖选择推定的转化胚。共同培养后一周内胚胎Genie Calli产生了第一个体细胞胚胎，并从四个月后从A. tumefaciens转化的愈伤组织再生。结果表明这种方法是最适合于转化两种品种的胚胎愈伤组织。

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《International Horticultural Congress on Science and Horticulture for People》|2012年||共7页
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作者
H.G. Cardoso; A.A. Peixe; A. Figueiredo; A. Baldé; M.S. Pais;
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中图分类 S663.1-532;
关键词
grapevine; genetic transformation; embryogénie calli; GUS; somatic embryos; particle bombardment; Agrobacterium tumefaciens;

机译：葡萄树;遗传转型;胚胎calli;gus;somic embryos;粒子轰炸;农杆菌突厥;

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1. Genetic transformation of V. vinifera - two alternative systems under evaluation. [J] . Cardoso H. G., Figueiredo A., Balde A., Acta Horticulturae . 2012,第931期

机译：V的遗传转化。 vinifera -正在评估的两个替代系统。
2. Genetic transformation of V. vinifera - two alternative systems under evaluation. [J] . Cardoso H. G., Figueiredo A., Balde A., Acta Horticulturae . 2012,第931期

机译：V的遗传转化。 vinifera -正在评估的两个替代系统。
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4. Genetic Transformation of V. vinifera - Two Alternative Systems under Evaluation [C] . H.G. Cardoso, A.A. Peixe, A. Figueiredo, International Horticultural Congress on Science and Horticulture for People . 2012

机译：V.Vinifera - 两种替代系统评估的遗传转化
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Genetic Transformation of V. vinifera - Two Alternative Systems under Evaluation

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