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首页> 外文会议>International Horticultural Congress on Science and Horticulture for People >Classic Methods and Biotechnical Tools in Lemon Breeding: Preliminary Results
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Classic Methods and Biotechnical Tools in Lemon Breeding: Preliminary Results

机译：柠檬育种的经典方法和生物技术工具：初步结果

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摘要

Spain is the most important lemon-producing country of the Mediterranean basin. The aim of lemon improvement is the selection of lemon trees giving excellent quality and productivity and the production of seedless lemons having the features demanded by consumers. In the spring of 2008 and 2009 we carried out 600 crossings between different lemon cultivars. We obtained about 34% fruit set. Four months after pollination fruit were harvested and immature embryos rescue was carried out. About 280 plants were recovered. The plants of 2008 were analysed by flow cytometry and ten triploid and one tetraploid plant were selected. The plants of polyembryonic seeds will be analysed by molecular markers to select zygotic plants. Also, we have made several sowings of seeds and about 1800 seedlings of different lemon cultivars were analysed by flow cytometry: 24 triploid and 10 tetraploid plants were selected. The triploid and tetraploid plants will be cultivated in the field for their evaluation and the tetraploid plants selected could be used to obtained triploid plants through crossing with diploid plants.
机译：西班牙是地中海盆地最重要的柠檬产区。柠檬改善的目的是选择柠檬树，提供出色的品质和生产力，生产具有消费者所需的特征的无籽柠檬。在2008年春天和2009年春天，我们在不同的柠檬品种之间进行了600个交叉。我们获得了大约34％的水果套装。收获授粉果实后四个月，进行未成熟的胚胎救援。回收了大约280株植物。通过流式细胞术分析2008的植物，并选择了10个三倍体和一个四倍体植物。将通过分子标记分析聚氧化锰片种子的植物来选择Zygotic植物。此外，我们已经通过流式细胞术分析了几种种子播种，并通过流式细胞术分析了约1800种不同的柠檬品种幼苗：选择24个三倍体和10个四倍体植物。三倍体和四倍体植物将在该领域中培养，用于评价，并且可以使用选择的四倍体植物通过与二倍体植物交叉来获得三倍体植物。

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