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Tylenchulus semipenetrans Biotype in Six Citrus-Producing Provinces of the Republic of South Africa

机译:Tylenchulululu semipenetrans在南非共和国的六个柑橘类省的生物型

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Due to the existence of nematode biotypes, trifoliate orange (Poncirus trifoliata) and its hybrids had unintended consequences in the management of the citrus nematode (Tylenchulus semipenetrans) in South Africa. The challenge could be traced to equating assumptions to empirical evidence. After the discovery of T. semipenetrans, the South Africa biotype was purported to be the Mediterranean biotype, with most growers switching over to Poncirus rootstock on virgin soil in order to suppress the citrus nematode. Failure of citrus orchards on P. trifoliata rootstocks on virgin soils resulted in the initiation of a national study to determine the South African citrus nematode biotype using three differential hosts, viz. rough lemon (Citrus jambhiri), P. trifoliata and olive (Olea europaea). An orchard was randomly selected in each of the six citrus-producing provinces, viz. Eastern Cape, Kwa-Zulu Natal, Limpopo, Mpumalanga, North West and Western Cape. Three-month-old differential host seedlings were inoculated with ca. 10000 J2s, arranged in a randomised complete block design with 15 replicates. At harvest, 120 days after inoculation, isolates from each province had the reproductive factor (RF = Pf/Pi) of less than one on olive, but greater than one on rough lemon and trifoliate orange. Reproductive factors less than one suggested that T. semipenetrans failed to reproduce and develop on olive, whereas that of greater than one suggested that the isolates reproduced and developed on the other two hosts. In conclusion, the differential host tests suggested that the citrus nematode biotype in South African was the Poncirus biotype, implying that the P. trifoliata rootstock which had been recommended for use in the suppression of the Mediterranean biotype was unsuitable.
机译:由于线虫生物型的存在,Trifoliate橙(Poncirus Trifoliata)及其杂种在南非柑橘Nematode(Tylenchulare Semipenetrans)的管理中受到意外后果。挑战可以追溯到与经验证据的假设等同。在发现神半管传出后,南非生物型被声称成为地中海生物型,大多数种植者转向维尔京土壤上的颅螺砧座,以抑制柑橘Nematode。柑橘果园在维珍土壤上的柑橘类植物失败导致国家研究开始使用三个差分宿主的南非柑橘Nematode Biotype。粗柠檬(柑橘jambhiri),p. trifoliata和橄榄(Olea europaea)。在六个柑橘生产省份,VIZ中随机选择了果园。东开普省,KWA-ZULU NATAL,LIMPOPO,MPUMALANGA,西北和西开普省。用CA接种了三个月历史的差分宿主幼苗。 10000 J2S,排列在随机完整的块设计中,具有15个重复。在收获时,接种后120天,每个省的分离物在橄榄上的生殖系数(RF = PF / PI)少于一个,但在粗柠檬和Trifoliate橙色上大于一个。少于人的生殖因素表明,T.半管蛋白酶未能在橄榄中繁殖和发展,而大于一个人建议在其他两个宿主上转载和开发的分离物。总之,差异宿主试验表明,南非的柑橘Nematode生物型是Poncirus生物型,这意味着推荐用于抑制地中海生物型以抑制地中海生物型的P. Trifoliata砧木是不合适的。

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