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Evaluation of Aseptic Techniques Used to Collect Bovine Milk Samples

机译:评估用于收集牛奶样品的无菌技术

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Bacterial culture of milk is one of the primary methods used to diagnose intramammary infections. Aseptic technique is thought to be vital when collecting milk samples for culture to minimize false positive results caused by bacteria on the teat end entering the sample during sample collection. Recommended practices for aseptically collecting milk include: gloved hands, pre-milking teat disinfection with a teat dip followed by drying with a single-use towel, scrubbing the teat end with gauze soaked in 70% alcohol, discarding a few streams of milk, and collecting the milk sample into a sterile vial1. While this approach is recommended, it has never been critically evaluated. Hence, the objective of this study was to determine if there were differences among teat end preparation techniques with regard to potential contamination of milk samples for culture.
机译:牛奶的细菌培养是用于诊断脑内感染的主要方法之一。在收集牛奶样品的培养物中,无菌技术被认为是至关重要的,以最小化由细菌在样品收集期间进入样品的细菌引起的假阳性结果。无菌收集牛奶的推荐做法包括:手套,用乳头浸渍浸渍乳酸消毒,然后用单用毛巾烘干,用纱布浸泡在70%酒精中,丢弃几块牛奶,将牛奶样品收集到无菌瓶中。虽然建议使用这种方法,但它从未被评估过。因此,本研究的目的是确定乳酸培养物潜在污染的乳酸结束制备技术是否存在差异。

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