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Fluorophore-labeled, Peptide-based Glycoclusters: Synthesis, Binding Properties for Lectins and Detection of Carbohydrate-binding Proteins in Cells

机译：荧光团标记，基于肽的糖蛋白：合成，凝集素的结合性质和细胞中碳水化合物结合蛋白的检测

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A facile and efficient solidphase synthesis of linear peptide-based glycoclusters with various valences and different spatial arrangements of the sugar ligands is described. The synthetic strategy includes 1) solid-phase synthesis of fluorophore-labeled, alkynecontaining peptides, 2) coupling of azide-linked, unprotected mono-, di-, and trisaccharides to the alkyne-conjugated peptides on a solid support by click chemistry, and 3) release of the fluorophore-labeled glycoclusters from the solid support. By using this methodology, 32 fluorescent glycoclusters with a valence ranging from 1 to 4 and different spatial arrangements of the sugar ligands were prepared. Lectinbinding properties of the glycoclusters were initially examined by using microarrays immobilized by various lectins.

机译：描述了具有各种价值的线性肽的糖蛋白酶的容纳和有效的固相合成和糖配体的不同空间布置。合成策略包括1）固相合成荧光团标记，烷烃含量的肽，2）偶氮连接，未受保护的单次，二 - 和三糖与单击化学的固体支持物上的炔蛋白缀合的肽偶联， 3）从固体载体中释放荧光标记的甘油蛋白。通过使用该方法，制备32种具有从1至4和糖配体的不同空间布置的价值的32个荧光糖蛋白。最初通过使用各种凝集素固定的微阵列来研究糖蛋白酶的凝集性性质。

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《International Carbohydrate Symposium》|2013年||共1页
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Xizhe Tian; Jaevoung Pai; Kyung-Hwa Baek;
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1. Fluorophore-labeled, Peptide-based Glycoclusters: Synthesis, Binding Properties for Lectins, and Detection of Carbohydrate-Binding Proteins in Cells [J] . Xizhe Tian, Jaeyoung Pai, Kyung-Hwa Baek, Chemistry, an Asian journal . 2011,第8期

机译：荧光团标记的，基于肽的糖簇：合成，凝集素的结合特性和细胞中糖结合蛋白的检测。
2. Glycocluster design for improved avidity and selectivity in blocking human lectin/plant toxin binding to glycoproteins and cells. [J] . Andre S, Lahmann M, Gabius HJ, Molecular pharmaceutics . 2010,第6期

机译：糖簇设计可提高亲和力和选择性，从而阻止人凝集素/植物毒素与糖蛋白和细胞的结合。
3. Perylenediimide-based glycoclusters as high affinity ligands of bacterial lectins: synthesis, binding studies and anti-adhesive properties [J] . Marion Donnier-Marechal, Nicolas Galanos, Teddy Grandjean, Organic & biomolecular chemistry . 2017,第47期

机译：基于ylene二酰亚胺的糖簇作为细菌凝集素的高亲和力配体：合成，结合研究和抗黏附特性
4. Fluorophore-labeled, Peptide-based Glycoclusters: Synthesis, Binding Properties for Lectins and Detection of Carbohydrate-binding Proteins in Cells [C] . Xizhe Tian, Jaevoung Pai, Kyung-Hwa Baek International Carbohydrate Symposium . 2013

机译：荧光团标记，基于肽的糖蛋白：合成，凝集素的结合性质和细胞中碳水化合物结合蛋白的检测
5. Gangliosides and complementary lectins in cell-cell recognition: Specific binding of neuronal gangliosides by myelin-associated glycoprotein. [D] . Yang, Lynda Jun-San. 1997

机译：神经节苷脂和互补凝集素在细胞识别中的作用：髓鞘相关糖蛋白对神经节神经节苷脂的特异性结合。
6. Endogenous lectins from cultured cells: subcellular localization of carbohydrate-binding protein 35 in 3T3 fibroblasts [O] . 1986

机译：来自培养细胞的内源性凝集素：3T3成纤维细胞中碳水化合物结合蛋白35的亚细胞定位
7. Carbohydrate-binding properties of an immobilized α-d-galactopyranosyl-binding protein (lectin) from the seeds of Bandeiraea simplicifolia [O] . Timothy T. Ross, Colleen E. Hayes, Irwin J. Goldstein 1976

机译：来自Bandeiraea Simplicifolia的种子的固定化α-D-半乳糖酰基结合蛋白（凝集素）的碳水化合物结合性质
8. Endothelial Cell Morphogenesis and Angiogenesis Mediated by Carbohydrate-Binding Protein Galectin-3 [R] . Raz, A. , Nangia-Makker, P. , Shekhar, P. V. 2003

机译：内皮细胞形态发生和血管生成由碳水化合物结合蛋白Galectin-3介导

Fluorophore-labeled, Peptide-based Glycoclusters: Synthesis, Binding Properties for Lectins and Detection of Carbohydrate-binding Proteins in Cells

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