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首页> 外文会议>International Carbohydrate Symposium >SCREENING METHODOLOGY FOR EVALUATION OF CHITIN DEACETYLASE LIBRARIES: APPLICATION TO VIBRIO CHOLERAE DEACETYLASE MUTANT LIBRARIES FOR ENGINEERED SPECIFICTY
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SCREENING METHODOLOGY FOR EVALUATION OF CHITIN DEACETYLASE LIBRARIES: APPLICATION TO VIBRIO CHOLERAE DEACETYLASE MUTANT LIBRARIES FOR ENGINEERED SPECIFICTY

机译：用于评价甲壳素脱乙酰化酶文库的筛选方法：施用霍乱胆拉肽脱乙酰化酶突变体文库的工程特征

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Directed evolution has proven to be a powerful and reliable strategy to modify and improve enzymes with regard to their catalytic efficiency, stability or substrate specificity [1]. The key to a successful directed evolution approach is the selection or screening technique to be applied. In order to evolve a certain property, diversity in the protein sequence is created and the screening effort depends on the library size, the larger the more distant are the new targets from the original properties. Even though the general trend was to increase the library size, sequential rounds of "focused" randomization and screening of smaller libraries has proven to be an interesting option in order to optimize the hit prospecting efforts (Iterative Saturation Mutagenesis and focused random mutagenesis strategies) [2].
机译：已被证明是一种强大而可靠的策略来修饰和改善其催化效率，稳定性或底物特异性的酶[1]。成功定向演化方法的关键是要应用的选择或筛选技术。为了演变某一性质，创建蛋白质序列中的多样性，筛选工作取决于图书馆尺寸，越来越远的是来自原始属性的新目标。尽管普遍趋势是增加图书馆规模，但顺序回合的“聚焦”随机化和筛选较小的图书馆已被证明是一个有趣的选择，以优化受到打击的勘探努力（迭代饱和诱变和聚焦随机诱变策略）[ 2]。

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