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Improved pose measurement and tracking system for motion correction of awake, unrestrained small animal SPECT imaging

机译:改进的姿势测量和跟踪系统,用于唤醒的运动校正,无拘无束的小动物SPECT成像

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An improved optical landmark-based pose measurement and tracking system has been developed to provide 3D animal pose data for a single photon emission computed tomography (SPECT) imaging system for awake, unanesthetized, unrestrained laboratory animals. The six degree of freedom animal position and orientation measurement data are time synchronized with the SPECT list mode data to provide for motion correction after the scan and before reconstruction. The tracking system employs infrared (IR) markers placed on the animal’s head along with synchronized, strobed IR LEDs to illuminate the reflectors and freeze motion while minimizing reflections. A new design trinocular stereo image acquisition system using IEEE 1394 CMOS cameras acquires images of the animal with markers contained within a transparent enclosure. The trinocular configuration provides improved accuracy, range of motion, and robustness over the binocular stereo previously used. Enhanced software detects obstructions, automatically segments the markers, rejects reflections, performs marker correspondence, and calculates the 3D pose of the animal’s head using image data from three cameras. The new hardware design provides more compact camera positioning with enhanced animal viewing through the 360 degree SPECT scan. This system has been implemented on a commercial gantry and tested using live mice and has been shown to be more reliable with higher accuracy than the previous system. Experimental results showing the improved motion tracking results are given.
机译:已经开发出改进的基于光学地标的姿态测量和跟踪系统,以提供用于唤醒,未测量的无拘无束的实验室动物的单个光子发射计算断层摄影(SPECT)成像系统的3D动物姿势数据。六个自由度动物位置和定向测量数据是与SPECT列表模式数据同步的时间,以在扫描之后提供运动校正和重建之前。跟踪系统采用红外线(IR)标记在动物的头部上以及同步,闪光的红外LED,以照亮反射器并冻结运动,同时最小化反射。使用IEEE 1394 CMOS相机的新设计三曲立体图像采集系统获取具有包含在透明外壳内的标记的动物的图像。三边形配置提供了先前使用的双目立体声的改进的精度,运动范围和鲁棒性。增强型软件检测障碍物,自动段标记,拒绝反射,执行标记对应,并使用来自三个摄像机的图像数据计算动物头的3D姿势。新的硬件设计提供了更紧凑的摄像头定位,通过360度Spect Scan进行了增强的动物。该系统已经在商业龙门架上实现并使用实时小鼠进行测试,并且已被证明比以前的系统更高的精度更可靠。给出了显示改进的运动跟踪结果的实验​​结果。

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