A Cybernetic Approach for Modelling of Complex Dynamic Expression in Recombinant Protein Production with Pichia pastoris

机译：一种用于匹配蛋白质生产复杂动态表达与PICHIA Pastoris的网络性途径

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This paper demonstrates the dynamic behaviour of recombinant pharmaceutical protein production with Pichia pastoris. Fully automated experiments with PAT applications deliver reproducible and reliable data. Based on this, complex expression and secretion courses are modelled with simple kinetic and cybernetic elements, including a small number of parameters. The model is useful for process analysis and optimization as well as for development of integrated production processes.

机译：本文展示了重组药物蛋白质产生的动态行为与Pichia Pastoris。具有PAT应用的全自动实验可提供可重复和可靠的数据。基于此，复杂的表达和分泌课程用简单的动力学和网络感染元件进行建模，包括少量参数。该模型可用于过程分析和优化以及综合生产过程的发展。

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《IFAC Symposium on Computer Applications in Biotechnology》|2010年||共6页
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Reiner Luttmann; Elisabeth Hukelmann; Andree Ellert; Ali Kazemi; Gesine Cornelissen;
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中图分类 TP39-53;
关键词
Recombinant proteins; Pichia pastoris; Automated cultivation; Atline protein detection; Modelling of protein expression and secretion;

机译：重组蛋白;pichia pastoris;自动培养;在蛋白质检测中;蛋白质表达和分泌的建模;

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1. Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact on Cell Physiology and Recombinant Production of Secreted Proteins [J] . Màrius Tomàs-Gamisans, Cristiane Conte Paim Andrade, Francisco Maresca, Applied Microbiology . 2020,第6期

机译：重组Pichia Pastoris中NADH激酶异位过度表达的氧化还原工程（Komagataella phaffii）：对细胞生理学和分泌蛋白质的重组产生的影响
2. Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by Komagataella (Pichia) pastoris [J] . Theron Chrispian W., Berrios Julio, Delvigne Frank, Applied Microbiology and Biotechnology . 2018,第1期

机译：将代谢建模和群体异质性分析集成为优化Komagataella（Pichia）巴甫氏菌的重组蛋白生成
3. Expression and One-Step Purification of Recombinant Proteins Using an Alternative Episomal Vector for the Expression of N-Tagged Heterologous Proteins in Pichia pastoris [J] . Cristiane Akemi Uchima, Manabu Arioka Bioscience, Biotechnology, and Biochemistry . 2012,第2期

机译：使用替代再生载体的重组蛋白的表达和一步纯化用于表达N标记的异源蛋白在Pichia Pastoris中的表达
4. A Cybernetic Approach for Modelling of Complex Dynamic Expression in Recombinant Protein Production with Pichia pastoris [C] . Reiner Luttmann, Elisabeth Hukelmann, Andree Ellert, IFAC Symposium on Computer Applications in Biotechnology . 2010

机译：一种用于匹配蛋白质生产复杂动态表达与PICHIA Pastoris的网络性途径
5. Optimizing production methods for artificial silk proteins through bioreactor and purification studies of recombinant proteins expressed from Pichia pastoris [D] . Ramey, Aaron Thomson 2006

机译：通过生物反应器优化人工丝蛋白的生产方法并纯化巴斯德毕赤酵母表达的重组蛋白
6. Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact on Cell Physiology and Recombinant Production of Secreted Proteins [O] . Màrius Tomàs-Gamisans, Cristiane Conte Paim Andrade, Francisco Maresca, 2020

机译：重组Pichia Pastoris中NADH激酶异位过表达的氧化还原工程（Komagataella phaffii）：对细胞生理学和分泌蛋白质重组产生的影响
7. Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris ( Komagataella phaffii ): Impact on Cell Physiology and Recombinant Production of Secreted Proteins [O] . Màrius Tomàs-Gamisans, Cristiane Conte Paim Andrade, Francisco Maresca, 2020

机译：重组Pichia Pastoris中NADH激酶异位过表达的氧化还原工程（Komagataella phaffii）：对细胞生理学和分泌蛋白质重组产生的影响

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A Cybernetic Approach for Modelling of Complex Dynamic Expression in Recombinant Protein Production with Pichia pastoris

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