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Preparation of Ethanolic Butterfly Pea Extract Using Microwave Assisted Extraction and Loaded Nanostructured Lipid Carriers: Evaluation of Antioxidant Potential for Stabilization of Fish Oil

机译:使用微波辅助提取和负载纳米结构脂质载体制备乙醇蝶豌豆提取物:评价鱼油稳定抗氧化潜力

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Fresh butterfly pea was extracted with 95% ethanol and analyzed the antioxidant activity using DPPH scavenging activity and total phenolic content. The total phenolic content of butterfly pea ethanolic extract (BE) was 26.90±1.12 mg gallic acid equivalents/g of extract and radical DPPH scavenging activity of ethanolic extract was 27.78±0.12 mg Trolox equivalents/g of extract. The ethanolic extract-loaded Nanostructured Lipid Carriers called BE-NLC was prepared by the high shear homogenization method using compritol 888 ATO, miglyol oil, poloxamer 188, extract and tween 80 and then determined the particle size, polydispersity index, zeta potential and entrapment efficiency. The results showed that the morphology of the BE-NLC was spherical. The entrapment efficiency of extract was 72.51±1.11% in NLC system. Antioxidant potential of BE and BE-NLC for stabilization of fish oil was evaluated and compared with butylated hydroxy toluene (BHT) as synthetic antioxidant. This antioxidant potential was evaluated by the estimation of peroxide values (PVs) of catfish oil by treated with natural antioxidants (BE and BE-NLC) and BHT by incubating at 65°C for a period of 12 days. The results revealed that BHT had higher antioxidant activity than BE and BE-NLC in fish oil. In addition, treatment containing BE-NLC and BHT in fish oil performed similar PVs after the 4 days. However, the antioxidant activity of BE-NLC was higher than that of BE in stabilization of fish oil.
机译:用95%乙醇提取新鲜的蝴蝶豌豆,并使用DPPH清除活性和总酚醛含量分析抗氧化活性。蝴蝶酸豌豆乙醇提取物的总酚含量为26.90±1.12mg加入酸等当量/ g萃取物和自由基DPPH清除活性的乙醇提取物为27.78±0.12mg Trolox当量/ g.通过使用包含含量的888 ATO,米甘油油,泊洛沙姆188,提取物和吐温80,通过高剪切均化方法制备称为BE-NLC的乙醇提取物纳米结构脂质载体,然后确定粒度,多分散指数,Zeta电位和夹紧效率。结果表明,BE-NLC的形态是球形的。 NLC系统中提取物的夹带效率为72.51±1.11%。评价抗氧化剂和BE-NLC用于稳定鱼油的稳定,与合成抗氧化剂的丁基化羟基甲苯(BHT)进行比较。通过用天然抗氧化剂(BE和BE-NLC)和BHT在65℃下孵育12天的时间,通过估计鲶鱼油的过氧化物值(PVS)来评价该抗氧化剂潜力。结果表明,BHT具有比鱼油的抗氧化活性更高,并且是NLC。此外,在4天后,含有BE-NLC和FES-NLC和BHT的处理进行了类似的PV。然而,BE-NLC的抗氧化活性高于鱼油稳定的抗氧化活性。

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