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首页> 外文会议>International Conference on the Development of Biomedical Engineering in Vietnam >Effect of Dfbutyryl-Camp (Dbc-AMP) and Follicle Stimulation Hormone (FSH) on Meiotic Competence of Porcine Oocytes Dyring In Vitro Matyration and Fertilization
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Effect of Dfbutyryl-Camp (Dbc-AMP) and Follicle Stimulation Hormone (FSH) on Meiotic Competence of Porcine Oocytes Dyring In Vitro Matyration and Fertilization

机译：DFBUTYRYL-CAMP（DBC-AMP）和卵泡刺激激素（FSH）对猪卵母细胞大豆卵母细胞大豆菌和施肥的影响

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In order to produce good mature oocyte supporting for in vitro fertilization (IVF) and enhancing the. developmental competence of embryo, dibutyryl cAMP (dbcAMP) and follicle stimulation hormone (FSH) were supplemented to the in vitro maturation (IVM) medium. This study investigated the effect of dbcAMP and FSH on meiotic competence after IVM and IVF parameter. First, we examined the concentrations (0.5 and 1.0 mM) of dbcAMP on the maturation of fully-grown porcine oocytes. There was no significant difference in maturation rate. However, cumulus expansion was remarkably higher in treatment with dbcAMP 1.0 mM than 0.5 mM and non-treatment group (P < 0.05). We then examined the combination effect of dbcAMP (1.0 mM) and FSH (0.01 and 0.02 IU/mL) on oocyte maturation followed subsequent in vitro fertilization. The results showed that oocytes cultured in basic IVM medium supplemented with dbcAMP 1.0 mM and FSH 0.01 IU/mL possessed both high maturation and normal fertilization rate compared to other groups significantly (P < 0.05).
机译：为了产生良好的成熟卵母细胞，支持体外施肥（IVF）并增强。胚胎的发育能力，Dibutyll阵营（DBCAMP）和卵泡刺激激素（FSH）的培养率（IVM）培养基。本研究调查了DBCAMP和FSH对IVM和IVF参数后减数分裂竞争力的影响。首先，我们检查了植物成熟的DBCAMP的浓度（0.5和1.0mm）。成熟率没有显着差异。然而，用DBCamp 1.0mm小于0.5mm和非处理组的Dbcamp处理非常高的浓度膨胀非常高（P <0.05）。然后，我们检查了DBCAMP（1.0mm）和FSH（0.01和0.02 IU / mL）对卵母细胞成熟的组合效果，然后在体外施肥中进行。结果表明，与DBCamp的基本IVM培养基中培养的卵母细胞培养为DBCamp 1.0mm和FSH 0.01 IU / mL，与其他组显着相比具有高成熟和正常施肥率（P <0.05）。

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