• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Biophotonics and Immune Responses >Study of photodynamic activity of hypericin and synthetic photosensitizers on haemolysis of erythrocytes in vitro
【24h】

Study of photodynamic activity of hypericin and synthetic photosensitizers on haemolysis of erythrocytes in vitro

机译：高蛋白和合成光敏剂在体外红细胞溶血中的光动力活性研究

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Influence of hypericin and synthetic (see manuscript for formula) on haemolysis of human erythrocytes was investigated. It was shown that both hypericin and synthetic porphyrins (TOEPyP and Zn-TOEPyP) did not cause haemolysis in the dark (24 hrs incubation), whereas Ag-TOEPyP leaded to haemolysis already after 40 min incubation, i.e. was cytotoxic. Hypericin (25 μM -125 μM) possessed haemolytic activity upon light exposure (visible spectrum, 30 mW/cm2, 5 min and more). Total haemolysis of erythrocytes was observed at 15 min exposure to light at all the investigated concentrations of hypericin. Dose-dependent haemolytic effect of TOEPyP and Zn-TOEPyP depending on light exposure time was also investigated. TOEPyP demonstrated considerably higher haemolytic activity compared to Zn-TOEPyP. Ag-TOEPyP demonstrated the weakest photodynamic activity. The effect of ascorbic acid on porphyrin-induced haemolysis was also revealed. Ascorbic acid at the concentration of 0.15 μM and above significantly increased haemolysis induced by hypericin, whereas at concentration of 0.75 μM and less it appeared to possess protective property. TOEPyP and Zn-TOEPyP did not demonstrate photodynamic properties in the presence of ascorbic acid (3.75 μM and more). The ascorbic acid at the concentrations below 3.75 μM did not have any influence on erythrocyte haemolysis induced by TOEPyP, while it increased haemolytic effect of Zn-TOEPyP. Thus, the full inhibition of photohaemolysis induced by (see manuscript for formula) by singlet oxygen quenchers - ascorbic acid and tryptophan - was shown. This testifies to the fact, that the photohaemolysis induced by these porphyrins is caused by Type II reactions.
机译：研究了高白素和合成（参见式）对人红细胞溶血的影响。结果表明，Hypericin和合成卟啉（Toeppy和Zn-Toepyp）在黑暗中没有引起溶血（24小时孵育），而Ag-Toeppy在40分钟孵育后已经过量溶于溶血，即是细胞毒性。高蛋白（25μm-125μm）在曝光时具有溶血活性（可见光谱，30mW / cm 2，5分钟和更多）。在15分钟内观察到红细胞的总溶血，以在所有研究的高霉素中接触光。还研究了根据浅曝光时间的Toeppyp和Zn-Toepyp的剂量依赖性溶血作用。与Zn-Toepyp相比，Toepyp显示出相当高的溶血活性。 Ag-Toepyp展示了最弱的光动力活动。还揭示了抗坏血酸对卟啉诱导的溶血性的影响。浓度为0.15μm的抗坏血酸显着增加了由金丝酰诱导的溶血溶解，而浓度为0.75μm，似乎具有保护性。 Toeppy和Zn-Toeppyp在存在抗坏血酸（3.75μm和更多）的情况下没有证明光动力学性质。低于3.75μm的浓度下的抗坏血酸对Toepyp诱导的红细胞溶血没有任何影响，而它增加了Zn-Toeppy的溶血作用。因此，示出了通过单次氧猝灭剂 - 抗坏血酸和色氨酸诱导（参见所述配方稿）诱导的光晕溶解的全面抑制。这证明了这些卟啉诱导的光晕溶解是由II型反应引起的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号