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Inheritance and stability of spinosad resistance in a laboratory strain of the tobacco budworm

机译：烟草芽膜实验室菌株纺丝体抗性的遗传与稳定性

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In 1996 and 1997 field collections of North Carolina tobacco budworm larvae, Heliothis virescens (R), were established as a laboratory (parental) strain. Larvae from this colony were repeatedly selected for spinosad resistance by topical applicationof technical spinosad (a mixture of spinosyns A and D) over 13 generations. Topical LD50s 18 days after treatment were 0.11 mu g of active ingredient per larva for the parental (susceptible) strain and 73.55 mu g per larva for generation (0) 19 of the selected strain. When the resistant and the susceptible strains were crossed, and the F1 (R X S) budworms then backcrossed to the resistant strain, spinosad resistance was found to be the result of a partially recessive, single gene. The F1 budworms wereestimated to be 4- to 5-fold resistant compared to the susceptible strain. The stability of resistance in the absence of immigration and exposure to spinosad for 5 generations in a cohort of the resistant strain was examined. The LD50 decreased by 1.4-fold compared to the resistant budworms. Comparing the developmental biology of the susceptible (parental) and resistant strains, resistant males developed a little slower as larvae and emerged as adults later than susceptible males and had slightly smaller 1 day old pupal wet weights. When susceptible (S) and resistant (R) moths of both sexes were allowed to mate freely in a mixed population (80 percent R X 20 percent S). the majority of the offspring (84.6 percent) were susceptible to spinosad. This indicates reduced reproductive competitiveness for the resistant strain.
机译：1996年和1997年和1997年北卡罗来纳州烟草芽虫幼虫，Heliothis virescens（R）的田间收集，被确定为实验室（父母）菌株。通过技术Spinosad（Spinosyns A和D的混合物）以上，重复选择来自该菌落的幼虫，用于局部适用于13代以上的局部纯化术。治疗后18天的局部LD50S为每只幼虫为0.11μg活性成分，用于治疗（易感）菌株，每种幼虫为73.55μg的选定菌株的产生（0）19。当抗抗性和敏感的菌株交叉时，并且F1（R X S）芽虫然后回交抗性菌株时，发现尖晶矩的抗性是部分隐性的单基因的结果。与易感菌株相比，F1芽虫均为4至5倍。检查了在没有移民和暴露于旋转菌株队队的旋流子组的抗性的耐受性的稳定性。与抗性芽虫相比，LD50降低1.4折。比较易感（父母）和抗性菌株的发育生物学，抗性雄性发育较慢的幼虫较慢，并在晚后作为成年人出现，而不是易感雄性，并且具有略微较小的1天蛹湿重量。当易感易感（r）蛾（R）蛾类的抗蛾类时被允许在混合种群中自由配合（80％r×20％s）。大多数后代（84.6％）易患锭肌肉。这表明对抗性菌株的生殖竞争力降低。

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