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首页> 外文会议>Beltwide Cotton Conferences >GERMPLASM EVALUATION OF COTTON ACCESSIONS FROM THE U.S. COTTON GERMPLASM COLLECTION, USDA-ARS (GOSSYPIUM HIRSUTUM L. LANDRACES OF MEXICO)
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GERMPLASM EVALUATION OF COTTON ACCESSIONS FROM THE U.S. COTTON GERMPLASM COLLECTION, USDA-ARS (GOSSYPIUM HIRSUTUM L. LANDRACES OF MEXICO)

机译：来自美国棉质种质收集，USDA-ARS（Gossypium Hirsutum L. Lateraces的Lateraces的墨西哥）的种质评价

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Future improvement of cotton relies on introgression of genetic variability from wild cotton resources. Characterizing, understanding and adding to cotton germplasm collections is important for future cotton improvement. A set of 434 Gossypium hirsutum landraces of Mexico from the U.S. Cotton Germplasm Collection was planted at Shafter, CA for phenotypic and genetic characterization of variability. Commonly used phenotypic characters were scored for each accession and tissue was bulked of each accession for DNA extraction. PCR based microsatellite markers from public sources and developed at Shafter are being used to describe genetic variation in the same accessions. A subset of accessions will be scored on individual plants for phenotypic and molecular marker variation to compare between and within accession variation. The combination of the two approaches will provide a better description of the variation in the collection, provide a template for future data collection and description of cotton germplasm, and may discover relationships between the markers and the phenotypic traits (i.e. linkage to genes). Preliminary results showed that significant variation still exists in the collection from the data collection. Diversity between and within accessions was the highest for accessions collected within the states of Guerrero, Yucatan, Oaxaca, Veracruz and Chiapas. The use of molecular markers is expected to reveal more variation not evident in the physical traits, and will become a valuable toolin the maintenance and utility of the diversity of the germplasm collection.
机译：棉花的未来改善依赖于野生棉资源的遗传变异累积。表征，理解和加入棉种质收集对于未来的棉花改善是重要的。来自美国棉种质收集的一套434个Gossymium Hirsutum Lateraces，CA种植型棉种质收集，用于表型和遗传表征的变异性。常用的表型特征被评分为每种加入，并且组织的每次加入都膨胀，用于DNA提取。来自公共来源的基于PCR基的微卫星标记，并在Shafter开发用于描述相同摘要的遗传变异。将在个体植物上进行探讨的一种探讨，用于在加入变异之间进行比较和在加入变异之间进行比较和分子标记变化。两种方法的组合将提供更好地描述集合中的变化，为未来数据收集和棉种质的描述提供了模板，并且可能发现标记物和表型性状之间的关系（即对基因的连接）。初步结果表明，从数据收集中的集合中仍存在显着的变化。在Guerrero，Yucatan，Oaxaca，Veracruz和Chiapas的州内收集的进入之间的分类是最高的。预计使用分子标记将揭示在物理特征中不明显的变异，并且将成为一种有价值的工具，该工具素的维护和效用的种质收集的多样性。

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